Development of antiviral biomaterials by naturally occurring polysaccharides 天然多糖類による抗ウイルス性バイオ材料の開発

博士論文

To develop a biomaterial with an influenza virus-adsorptive functionality, an alkyl curdlan sulfate was prepared by ionic interaction between a positively charged didodecyldimethyl ammonium bromide and a negatively charged sulfate group of curdlan sulfate, which has potent anti-HIV activity, and then coated on a membrane filter with a 1μm pore size by hydrophobic interaction with the long alkyl groups in the curdlan sulfate. The alkyl curdlan sulfate with the degree of alkylation (DOA) of 0.03 (1 didodecyldimethyl group/12 sugar residues with 36 hydroxyl or sulfate groups) showed potent anti-HIV activity in a 50% effective concentration (EC50) as low as 0.87 μg/ml (standard curdlan sulfate EC50 = 0.3 μg/ml), and the activity decreased with increasing DOA. A DOA higher than 0.1 (1 didodecyldimethyl group/three sugar residues with nine hydro xyl or sulfate groups) gave no anti-HIV activity. Although both curdlan sulfate and alkyl curdlan sulfates did not inhibited infection of MDCK cells by influenza viruses, the alkyl curdlan sulfate-coated