Suppression of the Rho/Rho-Kinase Pathway and Prevention of Cerebral Vasospasm by Combination Treatment with Statin and Fasudil After Subarachnoid Hemorrhage in Rabbit

著者

    • Naraoka, Masato
    • Munakata, Akira
    • Matsuda, Naoya
    • Shimamura, Norihito
    • Ohkuma, Hiroki

書誌事項

タイトル

Suppression of the Rho/Rho-Kinase Pathway and Prevention of Cerebral Vasospasm by Combination Treatment with Statin and Fasudil After Subarachnoid Hemorrhage in Rabbit

著者名

Naraoka, Masato

著者名

Munakata, Akira

著者名

Matsuda, Naoya

著者名

Shimamura, Norihito

著者名

Ohkuma, Hiroki

学位授与大学

弘前大学

取得学位

博士(医学)

学位授与番号

甲第1807号

学位授与年月日

2013-09-30

注記・抄録

The Rho/Rho-kinase pathway is considered important in the pathogenesis of sustained smooth muscle cell contraction during cerebral vasospasm after aneurysmal subarachnoid hemorrhage (SAH). The aims of this study were to investigate whether combination treatment, with pitavastatin as an inhibitor of RhoA and fasudil as an inhibitor of Rho-kinase, prevents the cerebral vasospasm. SAH was simulated using the double-hemorrhage rabbit model, and pitavastatin, or fasudil, or both (combination treatment) were administrated. The basilar artery (BA) cross-sectional area only in the combination treatment group was statistically larger than in the SAH group (p < 0.05). BA Rho-kinase, as measured by ELISA, was statistically reduced only in the combination treatment group compared with the SAH group (p < 0.05). In the other two treatment groups, pitavastatin or fasudil treatment group showed larger BA cross-sectional areas and lower value for BA Rho-kinase, but there were no statistically significant differences compared with the SAH group. The expression of endothelial nitric oxide synthase (eNOS), evaluated by immunohistochemistry in the pitavastatin group and the combination group, was higher than in the SAH group. Results indicate that combination treatment could extensively prevent cerebral vasospasm due to the synergic effect of combining pitavastatin and fasudil on the Rho/Rho-kinase pathway and on eNOS.

Open Access Article

開始ページ : 368

終了ページ : 374

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各種コード

  • NII論文ID(NAID)
    500000731241
  • NII著者ID(NRID)
    • 8000000747065
    • 8000000747066
    • 8000000747067
    • 8000000747068
    • 8000000747069
  • DOI(出版社)
  • DOI
  • 本文言語コード
    • en_US
  • データ提供元
    • 機関リポジトリ
    • NDLデジタルコレクション
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