Osteogenic matrix cell sheet transplantation enhances early tendon graft to bone tunnel healing in rabbits. 骨形成細胞シートを用いた家兎移植腱骨孔間の早期治癒促進

著者

    • Inagaki, Yusuke
    • Uematsu, Kota
    • Akahane, Manabu
    • Morita, Yusuke
    • Ogawa, Munehiro
    • Ueha, Tomoyuki
    • Shimizu, Takamasa
    • Kura, Tomohiko
    • Kawate, Kenji
    • Tanaka, Yasuhito

書誌事項

タイトル

Osteogenic matrix cell sheet transplantation enhances early tendon graft to bone tunnel healing in rabbits.

タイトル別名

骨形成細胞シートを用いた家兎移植腱骨孔間の早期治癒促進

著者名

Inagaki, Yusuke

著者名

Uematsu, Kota

著者名

Akahane, Manabu

著者名

Morita, Yusuke

著者名

Ogawa, Munehiro

著者名

Ueha, Tomoyuki

著者名

Shimizu, Takamasa

著者名

Kura, Tomohiko

著者名

Kawate, Kenji

著者名

Tanaka, Yasuhito

学位授与大学

奈良県立医科大学

取得学位

博士(医学)

学位授与番号

甲第608号

学位授与年月日

2013-11-27

注記・抄録

The objective of this study was to determine whether osteogenic matrix cell sheets (OMCS) could induce bone formation around grafted tendons, thereby enhancing early stage tendon to bone tunnel healing in skeletally mature male Japanese white rabbits. First, the osteogenic potential of rabbit OMCS was evaluated. Then, the OMCS were transplanted into the interface between the grafted tendon and the bone tunnel created at the tibia. Histological assessments and biomechanical tensile testing were performed after 3 weeks. The rabbit OMCS showed high alkaline phosphatase (ALP) activity, positive staining of ALP, and osteogenic potential when transplanted subcutaneously with beta tricalcium phosphate disks. Newly formed bony walls and positive collagen type I staining were seen around the grafted tendon with OMCS transplantation, whereas such bony walls were thinner or less frequent without OMCS transplantation. Micro-computed tomography images showed significantly higher bone volume in the OMCS transplantation group. The pullout strength was significantly higher with OMCS (0.74 ± 0.23 N/mm(2)) than without OMCS (0.58 ± 0.15 N/mm(2)). These results show that OMCS enhance early tendon to bone tunnel healing. This method can be applied to cases requiring early tendon to bone tunnel healing after ligament reconstruction surgery.

博士(医学)・甲第608号・平成25年11月27日

開始ページ : ID:842192

PubMed番号 : http://www.ncbi.nlm.nih.gov/pubmed/24106718

0アクセス

各種コード

  • NII論文ID(NAID)
    500000731525
  • NII著者ID(NRID)
    • 8000000907313
    • 8000000907314
    • 8000000907315
    • 8000000907316
    • 8000000907317
    • 8000000907318
    • 8000000907319
    • 8000000907320
    • 8000000907321
    • 8000000907322
  • DOI(出版社)
  • DOI
  • 本文言語コード
    • eng
  • データ提供元
    • 機関リポジトリ
    • NDLデジタルコレクション
ページトップへ