Osteogenic matrix cell sheet transplantation enhances early tendon graft to bone tunnel healing in rabbits. 骨形成細胞シートを用いた家兎移植腱骨孔間の早期治癒促進
Access this Article
Author
Bibliographic Information
- Title
-
Osteogenic matrix cell sheet transplantation enhances early tendon graft to bone tunnel healing in rabbits.
- Other Title
-
骨形成細胞シートを用いた家兎移植腱骨孔間の早期治癒促進
- Author
-
Inagaki, Yusuke
- Author
-
Uematsu, Kota
- Author
-
Akahane, Manabu
- Author
-
Morita, Yusuke
- Author
-
Ogawa, Munehiro
- Author
-
Ueha, Tomoyuki
- Author
-
Shimizu, Takamasa
- Author
-
Kura, Tomohiko
- Author
-
Kawate, Kenji
- Author
-
Tanaka, Yasuhito
- University
-
奈良県立医科大学
- Types of degree
-
博士（医学）
- Grant ID
-
甲第608号
- Degree year
-
2013-11-27
Note and Description
The objective of this study was to determine whether osteogenic matrix cell sheets (OMCS) could induce bone formation around grafted tendons, thereby enhancing early stage tendon to bone tunnel healing in skeletally mature male Japanese white rabbits. First, the osteogenic potential of rabbit OMCS was evaluated. Then, the OMCS were transplanted into the interface between the grafted tendon and the bone tunnel created at the tibia. Histological assessments and biomechanical tensile testing were performed after 3 weeks. The rabbit OMCS showed high alkaline phosphatase (ALP) activity, positive staining of ALP, and osteogenic potential when transplanted subcutaneously with beta tricalcium phosphate disks. Newly formed bony walls and positive collagen type I staining were seen around the grafted tendon with OMCS transplantation, whereas such bony walls were thinner or less frequent without OMCS transplantation. Micro-computed tomography images showed significantly higher bone volume in the OMCS transplantation group. The pullout strength was significantly higher with OMCS (0.74 ± 0.23 N/mm(2)) than without OMCS (0.58 ± 0.15 N/mm(2)). These results show that OMCS enhance early tendon to bone tunnel healing. This method can be applied to cases requiring early tendon to bone tunnel healing after ligament reconstruction surgery.
博士（医学）・甲第608号・平成25年11月27日
Copyright © 2013 Yusuke Inagaki et al. This is an open access article distributed under the Creative Commons Attribution License(https://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
identifier:BioMed research international Vol.2013 Article ID:842192
identifier:23146141
identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/2673
identifier:BioMed research international, 2013: Article ID:842192
開始ページ : Article ID:842192
