NHE-1 blockade reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricle. イソプロテレノール誘導肥大心においてNHE-1阻害薬は、ラット左心室の筋スライスのCa2+トランジェントを正常化する
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著者
書誌事項
- タイトル
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NHE-1 blockade reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricle.
- タイトル別名
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イソプロテレノール誘導肥大心においてNHE-1阻害薬は、ラット左心室の筋スライスのCa2+トランジェントを正常化する
- 著者名
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Hattori, Hiroshi
- 著者名
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Takeshita, Daisuke
- 著者名
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Takeuchi, Ayako
- 著者名
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Kim, Bongju
- 著者名
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Shibata, Munetaka
- 著者名
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Matsuoka, Satoshi
- 著者名
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Obata, Koji
- 著者名
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Mitsuyama, Shinichi
- 著者名
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Zhang, Guo-Xing
- 著者名
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Takaki, Miyako
- 学位授与大学
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奈良県立医科大学
- 取得学位
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博士(医学)
- 学位授与番号
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甲第618号
- 学位授与年月日
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2012-03-09
注記・抄録
We previously reported that left ventricular (LV) slices from isoproterenol (ISO)-induced hypertrophied rat hearts showed an increase of energy expenditure due to remodeling of Ca2+ handling in excitation–contraction coupling, i.e., suppressed SERCA2a activity and enhanced Na+/Ca2+exchanger-1 (NCX-1) activity. Na+/H+ exchanger-1 (NHE-1) inhibitor (NHEI) has been demonstrated to exert beneficial effects in the development of cardiac remodeling. We hypothesized that a novel NHE-1 selective inhibitor, BIIB723 prevents remodeling of Ca2+ handling in LV slices of ISO-induced hypertrophied rat hearts mediated by inhibiting NCX-1 activity. The significant shortening in duration of multi-cellular Ca2+ transient in ISO group was normalized in ISO + BIIB723 group. The significant increase in amplitude of multi-cellular Ca2+ waves (CaW) generated at high [Ca2+]o of LV slices in ISO group was also normalized in ISO + BIIB723 group. However, the enhanced NCX-1 activity was not antagonized by BIIB723. We recently reported that ISO-induced down-regulation of a Ca2+ handling protein, SERCA2a, was normalized by BIIB723. Therefore, it seems likely that BIIB723 normalized shortened multi-cellular Ca2+ transient duration and increased CaW amplitude in LV slices mediated via normalization of SERCA2a activity. Furthermore, the results presented here suggest the multi-cellular Ca2+ transient duration and CaW amplitude in LV slices might be better indices reflecting SERCA2a activity than SERCA2a protein expression level.
博士(医学)・甲618号・平成26年3月17日
identifier:Biochemical and biophysical research communications Vol.419 No.2 p.431-435
identifier:0006291X
identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/2703
identifier:Biochemical and biophysical research communications, 419(2): 431-435
開始ページ : 431
終了ページ : 435