Gene Expression of Type I and Type III Collagen by Mechanical Stretch in Anterior Cruciate Liganment Cells
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- Kim Sung-Gon
- Department of Orthopaedic Surgery, Juntendo University
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- Akaike Toshihiro
- 2Department of Biomolecular Engineering, Tokyo Institute of Technology
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- Sasagawa Tadashi
- 2Department of Biomolecular Engineering, Tokyo Institute of Technology
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- Atomi Yoriko
- 3Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
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- Kurosawa Hisashi
- Department of Orthopaedic Surgery, Juntendo University
書誌事項
- タイトル別名
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- Gene Expression of Type I and Type III Collagen by Mechanical Stretch in Anterior Cruciate Ligament Cells
- Gene Expression of Type 1 and Type 3 Collagen by Mechanical Stretch in Anterior Cruciate Liganment Cells
この論文をさがす
抄録
Mechanical stretch affects the healing and remodeling process of the anterior cruciate ligament (ACL) after surgery in important ways. In this study, the effects of mechanical stress on gene expression of type I and III collagen by cultured human ACL cells and roles of transforming growth factor (TGF)-β1 in the regulation of mechanical strain-induced gene expression were investigated. Uniaxial cyclic stretch was applied on ACL cells at 10 cycles/min with 10% length stretch for 24 h. mRNA expression of the type I and type III collagen was increased by the cyclic stretch. TGF-β1 protein in the cell culture supernatant was also increased by the stretch. In the presence of anti-TGF-β1 antibody, stretch-induced increase in type I and type III mRNA expression was markedly ablated. The results suggest that the stretch-induced mRNA expression of the type I and type III collagen is mediated via an autocrine mechanism of TGF-β1 released from ligament cells.<br>
収録刊行物
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- Cell Structure and Function
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Cell Structure and Function 27 (3), 139-144, 2002
日本細胞生物学会
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詳細情報
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- CRID
- 1390001204694771200
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- NII論文ID
- 130004137443
- 50000617078
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- NII書誌ID
- AA0060007X
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- COI
- 1:CAS:528:DC%2BD38Xns1ahtrk%3D
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- ISSN
- 13473700
- 03867196
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- NDL書誌ID
- 6291470
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- PubMed
- 12207044
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可