Simultaneous Optical and Electrical Recording of a Single Ion-Channel.

• Ide Toru

  Single Molecule Processes Project, International Cooperative Research Project, Japan Science and Technology Corporation

• Takeuchi Yuko

• Aoki Takaaki

• Yanagida Toshio

抄録

In recent years, the single-molecule imaging technique has proven to be a valuable tool in solving many basic problems in biophysics. The technique used to measure single-molecule functions was initially developed to study electrophysiological properties of channel proteins. However, the technology to visualize single channels at work has not received as much attention. In this study, we have for the first time, simultaneously measured the optical and electrical properties of single-channel proteins. The large conductance calcium-activated potassium channel (BK-channel) labeled with fluorescent dye molecules was incorporated into a planar bilayer membrane and the fluorescent image captured with a total internal reflection fluorescence microscope simultaneously with single-channel current recording. This innovative technology will greatly advance the study of channel proteins as well as signal transduction processes that involve ion permeation processes.<br>

収録刊行物

• The Japanese Journal of Physiology

  The Japanese Journal of Physiology 52 (5), 429-434, 2002

  一般社団法人　日本生理学会

キーワード

• bilayer
• single-channel
• BK-channel
• single-molecule
• imaging

詳細情報

• CRID: 1390282680019934592
• NII論文ID: 130004435840
• NII論文ID: 50000726068
• NII書誌ID: AA00691224
• DOI: 10.2170/jjphysiol.52.429
• COI: 1:CAS:528:DC%2BD3sXhtlCqsLs%3D
• ISSN: 18811396
• ISSN: 0021521X
• NDL書誌ID: 6408738
• PubMed: 12533247
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6408738
• Web Site: https://search.jamas.or.jp/link/ui/2003165206
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可