Acidic Amino Acid Transport Characteristics of a Newly Developed Conditionally Immortalized Rat Type 2 Astrocyte Cell Line (TR-AST).

• Tetsuka Kazuhiro

  Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University

• Hosoya Ken-ichi

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University CREST of Japan Science and Technology Corporation (JST)

• Ohtsuki Sumio

  Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University CREST of Japan Science and Technology Corporation (JST) New Industry Creation Hatchery Center, Tohoku University

• Takanaga Hitomi

  Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University CREST of Japan Science and Technology Corporation (JST) New Industry Creation Hatchery Center, Tohoku University

• Yanai Nobuaki

  Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University

• Ueda Masatsugu

  YS New Technology Institute Inc.

• Obinata Masuo

  CREST of Japan Science and Technology Corporation (JST) Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University

• Terasaki Tetsuya

  Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University CREST of Japan Science and Technology Corporation (JST) New Industry Creation Hatchery Center, Tohoku University

抄録

To characterize acidic amino acid transport in type 2 astrocytes, we established conditionally immortalized rat astrocyte cell lines (TR-AST) from newly developed transgenic rats harboring temperature-sensitive SV40 large T-antigen gene. TR-AST exhibited positive immunostaining for anti-GFAP antibody and A2B5 antibody, characteristics associated with type 2 astrocytes, and expressed glutamine synthetase. Acidic amino acid transporters, GLT-1 and system x_C^-, which consists of xCT and 4F2hc, were expressed in all TR-ASTs by RT-PCR. On the other hand, GLAST expression was found in TR-AST3 and 5. The characteristics of [³H]L-glutamic acid (L-Glu) uptake by TR-AST5 include an Na⁺-dependent and Na⁺-independent manner, concentration-dependence, and inhibition by L-aspartic acid (L-Asp) and D-aspartic acid (D-Asp). The corresponding Michaelis-Menten constants for the Na⁺-dependent and Na⁺-independent process were 36.3 μM and 155 μM, respectively. [³H]L-Asp and [³H]D-Asp uptake by TR-AST5 had an Na⁺-dependent and Na⁺-independent manner. This study demonstrated that GLT-1, system x_C^-, and GLAST were expressed in TR-AST, which has the characteristics of type 2 astrocytes and is able to transport acidic amino acids.

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 26 (4), 197-203, 2001

  日本細胞生物学会

キーワード

• acidic amino acid
• GLT-1
• GLAST
• system x_C^-
• transport
• type 2 astrocyte

詳細情報

• CRID: 1390282679674074624
• NII論文ID: 130004137393
• NII論文ID: 30023110157
• NII論文ID: 50000990437
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.26.197
• COI: 1:CAS:528:DC%2BD3MXosVWqs7k%3D
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 5954323
• PubMed: 11699636
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5954323
• Web Site: http://www.jstage.jst.go.jp/article/csf/26/4/26_4_197/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2002131884
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可