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- H.C. Birnboim
- Laboratoire de Génétique Moléculaire, Institut de Recherche en Biologie Moleculaire, F-75221 Paris Cedex 05, France
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- J. Doly
- Laboratoire de Génétique Moléculaire, Institut de Recherche en Biologie Moleculaire, F-75221 Paris Cedex 05, France
抄録
<jats:title>Abstract</jats:title> <jats:p>A procedure for extracting plasmid DNA from bacterial cells 1s described. The method 1s simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.</jats:p>
収録刊行物
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- Nucleic Acids Research
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Nucleic Acids Research 7 (6), 1513-1523, 1979-11-24
Oxford University Press (OUP)
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キーワード
詳細情報 詳細情報について
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- CRID
- 1360292620330675072
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- NII論文ID
- 80000395302
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- ISSN
- 13624962
- 03051048
- http://id.crossref.org/issn/03051048
- http://id.crossref.org/issn/01980238
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