The Role in CaCO₃ Crystallization of an Acid Ca²⁺‐Binding Polysaccharide Associated with Coccoliths of <i>Emiliania huxleyi</i>

• Anton H. BORMAN

• Elisabeth W. de JONG

• Marlies HUIZINGA

• Dik J. KOK

• Peter WESTBROEK

• Leendert BOSCH

抄録

Coccoliths of the unicellular marine alga Emiliania huxleyi are formed intracellularly in a specialized vesicle. Closely associated with the CaCO 3 crystals of the coccoliths is an acid Ca 2+ ‐binding polysaccharide. The latter is considered to fulfil a regulatory function in CaCO 3 crystallization. In this study it is demonstrated that the coccolith polysaccharide is able to inhibit CaCO 3 precipitation in vitro . The degree of inhibition is dependent on the nature of the cations bound to the acid groups of the polysaccharide. After substitution of Na + by Ca 2+ ions the polysaccharide is far less effective in inhibiting CaCO 3 precipitation. The coccolith polysaccharide contains two types of acid groups: uronic acids and sulphate esters. Only the uronic acids are responsible for the inhibition of CaCO 3 precipitation. Desulphated polysaccharide inhibits precipitation to the same extent as the native molecule whereas the carboxyl‐reduced polysaccharide is unable to block CaCO 3 crystallization. Inhibition of CaCO 3 precipitation can be suppressed by the presence of ethanol. Presumably the conformation of the molecule is altered under these conditions. Alginic acid [poly(mannuronic‐iduronic acid)] and poly(galacturonic acid) also inhibit CaCO 3 precipitation. Inhibition of precipitation is effected by the coccolith polysaccharide and alginic acid at comparable concentrations. The concentration of poly(galacturonic acid) needed to obtain the same result is about 20‐times higher. Contrary to the coccolith polysaccharide, both poly(uronic acid)s inhibit CaCO 3 precipitation in the absence as well as in the presence of ethanol. These results suggest that conformational changes of the coccolith polysaccharide may play a role in CaCO 3 crystallization in vivo .

収録刊行物

• European Journal of Biochemistry

  European Journal of Biochemistry 129 (1), 179-183, 1982-12

  Wiley

キーワード

• Biochemistry

詳細情報

• CRID: 1360292619333028608
• NII論文ID: 80001453424
• DOI: 10.1111/j.1432-1033.1982.tb07037.x
• ISSN: 14321033
• ISSN: 00142956
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1982.tb07037.x
• Web Site: https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1982.tb07037.x
• データソース種別:
  • Crossref
  • CiNii Articles