Superinduction of c- <i>fos</i> by Nerve Growth Factor in the Presence of Peripherally Active Benzodiazepines

• Tom Curran

  Department of Molecular Oncology and Developmental Biology, Hoffmann-La Roche, Inc., Nutley, New Jersey 07110

• James I. Morgan

  Department of Physiological Chemistry and Pharmacology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110

抄録

Alterations in proto-oncogene expression after stimulation of rat pheochromocytoma (PC12) cells by nerve growth factor (NGF) have been investigated. A specific stimulation of c- fos messenger RNA and protein was detected 30 minutes after treatment. This induction was enhanced more than 100-fold in the presence of peripherally active benzodiazepines. The effect was specific as very little change was observed in the levels of c- ras Ha , c- ras Ki , c- myc , and N- myc messenger RNA's. Under the conditions used here, NGF treatment ultimately results in neurite outgrowth, with a reduction or cessation of cell division. Thus, stimulation of the c- fos gene in this system appeared to be associated with differentiation and not with cellular proliferation. The effect of benzodiazepines was stereospecific and represents a novel action of these compounds at the level of gene expression.

収録刊行物

• Science

  Science 229 (4719), 1265-1268, 1985-09-20

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360855569708150272
• NII論文ID: 80002609793
• DOI: 10.1126/science.4035354
• ISSN: 10959203
• ISSN: 00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.4035354
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