Recombinant Human Tumor Necrosis Factor-α: Effects on Proliferation of Normal and Transformed Cells in Vitro

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  • Barry J. Sugarman
    Department of Pharmacological Sciences, Genentech, Inc., South San Francisco, California 94080
  • Bharat B. Aggarwal
    Department of Protein Biochemistry, Genentech, Inc., South San Francisco, California 94080
  • Philip E. Hass
    Department of Protein Biochemistry, Genentech, Inc., South San Francisco, California 94080
  • Irene S. Figari
    Department of Pharmacological Sciences, Genentech, Inc., South San Francisco, California 94080
  • Michael A. Palladino
    Department of Pharmacological Sciences, Genentech, Inc., South San Francisco, California 94080
  • H. Michael Shepard
    Department of Pharmacological Sciences, Genentech, Inc., South San Francisco, California 94080

Abstract

<jats:p>Modulation of the growth of human and murine cell lines in vitro by recombinant human tumor necrosis factor-α (rTNF-α) and recombinant human interferon-γ (rIFN-γ) was investigated. rTNF-α had cytostatic or cytolytic effects on only some tumor cell lines. When administered together with rIFN-γ, rTNF-α showed enhanced antiproliferative effects on a subset of the cell lines tested. In contrast to its effects on sensitive tumor cells, rTNF-α augmented the growth of normal diploid fibroblasts. Variations in the proliferative response induced by rTNF-α were apparently not due to differences in either the number of binding sites per cell or their affinity for rTNF-α. These observations indicate that the effects of rTNF-α on cell growth are not limited to tumor cells, but rather that this protein may have a broad spectrum of activities in vivo.</jats:p>

Journal

  • Science

    Science 230 (4728), 943-945, 1985-11-22

    American Association for the Advancement of Science (AAAS)

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