The Product of the Human c- <i>erb</i> B-2 Gene: a 185-Kilodalton Glycoprotein with Tyrosine Kinase Activity

DOI Web Site 被引用文献21件
  • Tetsu Akiyama
    Department of Biochemistry, Meiji College of Pharmacy, 1-35-23 Nozawa, Setagaya-ku Tokyo 154, Japan.
  • Chie Sudo
    Department of Biochemistry, Meiji College of Pharmacy, 1-35-23 Nozawa, Setagaya-ku Tokyo 154, Japan.
  • Hiroshi Ogawara
    Department of Biochemistry, Meiji College of Pharmacy, 1-35-23 Nozawa, Setagaya-ku Tokyo 154, Japan.
  • Kumao Toyoshima
    Laboratory of Oncology, Institute of Medical Science, University of Tokyo, 4-6-1 Shiroganedai, Minato-ku Tokyo 108, Japan.
  • Tadashi Yamamoto
    Laboratory of Oncology, Institute of Medical Science, University of Tokyo, 4-6-1 Shiroganedai, Minato-ku Tokyo 108, Japan.

抄録

<jats:p> Antibodies were raised against a synthetic peptide corresponding to 14 amino acid residues at the COOH-terminus of a protein deduced from the human c- <jats:italic>erb</jats:italic> B-2 nucleotide sequence. These antibodies immunoprecipitated a 185-kilodalton glycoprotein from MKN-7 adenocarcinoma cells. Incubation of the immunoprecipitates with (γ- <jats:sup>32</jats:sup> )ATP resulted in the phosphorylation of this protein on tyrosine residues. These results indicate that the human c- <jats:italic>erb</jats:italic> B-2 gene product is the 185-kilodalton glycoprotein that is associated with tyrosine kinase activity. Although the c- <jats:italic>erb</jats:italic> B-2 protein was predicted to encode a protein very similar to epidermal growth factor (EGF) receptor, EGF did not stimulate this kinase activity either in vivo or in vitro. </jats:p>

収録刊行物

  • Science

    Science 232 (4758), 1644-1646, 1986-06-27

    American Association for the Advancement of Science (AAAS)

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