Mouse Embryonic Stem Cells and Reporter Constructs to Detect Developmentally Regulated Genes

DOI Web Site 被引用文献29件
  • Achim Gossler
    Mount Sinai Hospital Research Institute, Division of Molecular and Developmental Biology, Toronto, Canada MSG 1X5.
  • Alexandra L. Joyner
    Mount Sinai Hospital Research Institute, Division of Molecular and Developmental Biology, Toronto, Canada MSG 1X5.
  • Janet Rossant
    Mount Sinai Hospital Research Institute, Division of Molecular and Developmental Biology, Toronto, Canada MSG 1X5.
  • William C. Skarnes
    Department of Medical Genetics University of Toronto, Toronto, Canada.

抄録

<jats:p> A strategy was devised for identifying regions of the mouse genome that are transcriptionally active in a temporally and spatially restricted manner during development. The approach is based on the introduction into embryonic stem cells of two types of <jats:italic>lacZ</jats:italic> reporter constructs that can be activated by flanking mouse genomic sequences. Embryonic stem cells containing the <jats:italic>lacZ</jats:italic> constructs were used to produce chimaeric mice. Developmental regulation of <jats:italic>lacZ</jats:italic> expression occurred at a high frequency. Molecular cloning of the flanking endogenous genes and introduction of these potential insertional mutations into the mouse germ line should provide an efficient means of identifying and mutating novel genes important for the control of mammalian development. </jats:p>

収録刊行物

  • Science

    Science 244 (4903), 463-465, 1989-04-28

    American Association for the Advancement of Science (AAAS)

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