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- P J Casey
- Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
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- P A Solski
- Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
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- C J Der
- Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
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- J E Buss
- Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
抄録
<jats:p>Association of oncogenic ras proteins with cellular membranes appears to be a crucial step in transformation, ras is synthesized as a cytosolic precursor, which is processed to a mature form that localizes to the plasma membrane. This processing involves, in part, a conserved sequence, Cys-Ali-Ali-Xaa (in which Ali is an amino acid with an aliphatic side chain and Xaa is any amino acid), at the COOH terminus of ras proteins. Yeast a-factor mating hormone precursor also possesses a COOH-terminal Cys-Ali-Ali-Xaa sequence. However, while the COOH-terminal cysteine has been implicated as a site of palmitoylation of ras proteins, in mature a-type mating factor this residue is modified by an isoprenoid, a farnesyl moiety. We asked whether the Cys-Ali-Ali-Xaa sequence signaled different modifications for the yeast peptides (farnesylation) than for ras proteins (palmitoylation) or whether ras proteins were similar to the mating factors and contained a previously undiscovered isoprenoid. We report here that the processing of ras proteins involves addition of a farnesyl moiety, apparently at the COOH-terminal cysteine analogous to the cysteine modified in the yeast peptides, and that farnesylation may be important for membrane association and transforming activity of ras proteins.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 86 (21), 8323-8327, 1989-11
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1360011145204338048
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- NII論文ID
- 80004839916
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- ISSN
- 10916490
- 00278424
- http://id.crossref.org/issn/00278424
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- データソース種別
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