ミクロ２次元電気泳動法による歯肉溝浸出液の蛋白質分析

DOI 被引用文献3件
  • 和田 緑
    昭和大学歯学部第3歯科補綴学教室
  • 芝 紀代子
    東京医科歯科大学医学部附属病院検査部生化学
  • 芝 〓彦
    昭和大学歯学部第3歯科補綴学教室

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  • Analysis of gingival fluid proteins by microscale two-dimensional electrophoresis.

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The analysis of gingival fluid proteins was performed by the combinative method of microscale two-dimensional electrophoresis with the highly sensitive silvers taining, which was possible to measure a minute amount of sample. 31-55 protein spots were detected, and 14 proteins of them were identified by electroblotting-immunochemical staining. As compared gingival fluid with mixed saliva and serum, the spots of isoelectric point of pI 5.2-5.5 and MW 130, 000-140, 000 were characteristic of gingival fluid. In the quantitative comparison, the IgA, ceruloplasmin and α2-HS glycoprotein spots were more deeply stained on the serum, while IgG and albumin spots were more deeply stained on the gingival fluid. We also demonstrated the presence of a haptoglobin with different pI position in gingival fluid, and recognized that the haptoglobin-hemoglobin complex observed in alkaline side were present in the advanced inflammatory conditions. We would like to conclude that a complex of haptoglobin and hemoglobin is closely related to inflammatory responses.

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