Interleukin 2 gene transfer into tumor cells abrogates tumorigenicity and induces protective immunity.

• B Gansbacher

  Department of Hematology/Lymphoma, Memorial-Sloan Kettering Cancer Center, New York, New York 10021.

• K Zier

  Department of Hematology/Lymphoma, Memorial-Sloan Kettering Cancer Center, New York, New York 10021.

• B Daniels

  Department of Hematology/Lymphoma, Memorial-Sloan Kettering Cancer Center, New York, New York 10021.

• K Cronin

  Department of Hematology/Lymphoma, Memorial-Sloan Kettering Cancer Center, New York, New York 10021.

• R Bannerji

  Department of Hematology/Lymphoma, Memorial-Sloan Kettering Cancer Center, New York, New York 10021.

• E Gilboa

  Department of Hematology/Lymphoma, Memorial-Sloan Kettering Cancer Center, New York, New York 10021.

抄録

To study the effects of localized secretion of cytokines on tumor progression, the gene for human interleukin 2 (IL-2) was introduced via retroviral vectors into CMS-5 cells, a weakly immunogenic mouse fibrosarcoma cell line of BALB/c origin. Secretion of low levels of IL-2 from the tumor cells abrogated their tumorigenicity and induced a long-lasting protective immune response against a challenge with a tumorigenic dose of parental CMS-5 cells. Co-injection of IL-2-producing CMS-5 cells with unmodified tumor cells inhibited tumor formation even when highly tumorigenic doses of CMS-5 cells were used. Cytolytic activity in mice injected with parental CMS-5 cells was transient and was greatly diminished 3 wk after injection, as commonly observed in tumor-bearing animals. However, in mice injected with IL-2-producing cells, tumor-specific cytolytic activity persisted at high levels for the duration of the observation period (at least 75 d). High levels of tumor-specific cytolytic activity could also be detected in parental CMS-5 tumor-bearing animals 18 d after inoculation with tumor cells, if IL-2-producing CMS-5 cells but not unmodified parental tumor cells were used as targets. These studies highlight the potential advantages of localized secretion of cytokines mediated via gene transfer to induce potent anti-tumor immune responses.

収録刊行物

• The Journal of experimental medicine

  The Journal of experimental medicine 172 (4), 1217-1224, 1990-10-01

  Rockefeller University Press

キーワード

• Immunology
• Immunology and Allergy

詳細情報

• CRID: 1361981470693243904
• NII論文ID: 80005529057
• DOI: 10.1084/jem.172.4.1217
• ISSN: 15409538
• ISSN: 00221007
• Web Site: https://rupress.org/jem/article-pdf/172/4/1217/1671531/1217.pdf
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