Phylogenetic relationships among species of <i>Saccharomyces</i>, <i>Schizosaccharomyces</i>, <i>Debaryomyces</i> and <i>Schwanniomyces</i> determined from partial ribosomal RNA sequences

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<jats:title>Abstract</jats:title><jats:p>Species of the genera <jats:italic>Saccharomyces</jats:italic>, <jats:italic>Schizosaccharomyces</jats:italic>, <jats:italic>Debaryomyces</jats:italic> and <jats:italic>Schwanniomyces</jats:italic> were compared from their extent of divergence in three regions from small (18S) and large (25S) subunit ribosomal RNAs comprising a total of 900 nucleotides. With the exception of the closely related <jats:italic>Saccharomyces bayanus</jats:italic> and <jats:italic>S. pastorianus</jats:italic>, which appear to have identical sequences, all other species could be distinguished by nucleotide differences in a variable region of the large subunit, and genus‐specific nucleotides were discernible in all three regions. The taxon <jats:italic>D. tamarii</jats:italic> differed markedly from other species and is excluded from <jats:italic>Debaryomyces</jats:italic>. By contrast, <jats:italic>Schwanniomyces occidentalis</jats:italic> showed few nucleotide differences with <jats:italic>Debaryomyces</jats:italic> spp. and its transfer to <jats:italic>Debaryomyces</jats:italic> is proposed. <jats:italic>Schizosaccharomyces</jats:italic> proved to be somewhat more divergent than <jats:italic>Saccharomyces</jats:italic> and <jats:italic>Debaryomyces</jats:italic>, but species differences appear insufficient for dividing the genus. Some of the factors influencing estimates of phylogenetic distances from rRNA sequences are discussed.</jats:p>

収録刊行物

  • Yeast

    Yeast 7 (1), 61-72, 1991-01

    Wiley

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