Involvement of ras p21 protein in signal-transduction pathways from interleukin 2, interleukin 3, and granulocyte/macrophage colony-stimulating factor, but not from interleukin 4.

• T Satoh

  DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304-1104.

• M Nakafuku

  DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304-1104.

• A Miyajima

  DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304-1104.

• Y Kaziro

  DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304-1104.

抄録

The protooncogene ras acts as a component of signal-transduction networks in many kinds of cells. The ras gene product (p21) is a GTP-binding protein, and the activity of the protein is regulated by bound GDP/GTP. Recent studies have shown that a certain class of growth factors stimulates the formation of active p21-GTP complexes in fibroblasts and that oncogene products with enhanced tyrosine kinase activities have a similar effect on ras p21. We have measured the ratio of active GTP-bound p21 to total p21 in several lymphoid and myeloid cell lines in order to understand the role of ras in the proliferation of these cells. Interleukin 2 (IL-2), IL-3, and granulocyte/macrophage colony-stimulating factor (GM-CSF) enhance the formation of the active p21.GTP, whereas IL-4 has no effect on p21-bound GDP/GTP. These results strongly suggest that ras p21 acts as a transducer of signals from IL-2, IL-3, and GM-CSF, but not from IL-4.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 88 (8), 3314-3318, 1991-04-15

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361981470745709824
• NII論文ID: 80005845868
• DOI: 10.1073/pnas.88.8.3314
• ISSN: 10916490
• ISSN: 00278424
• ISSN: http://id.crossref.org/issn/00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.88.8.3314
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