書誌事項
- タイトル別名
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- Study on eosinophil cationic protein(ECP) and arylsulfatase B in nasal secretions and sera from patients with nasal allergy.
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Recently four tissue toxic proteins namely major basic protein (MBP), eosinophil peroxydase (EPO), eosinophil-derived neurotoxin (EDN), and eosinophil cationic protein (ECP) were found in eosinophilic leucocytes. Although the characteristics of these proteins concerning tissue damage in the local site of type I allergic reaction have been investigated mainly in lower respiratory tract, the actual clinico-pathological roles of these proteins in nasal allergy are not clarified. Contrary, eosinophils also have histaminase, arylsulfatase, phospholipase D, which are considered to act on a negative feedback mechanism in allergic reaction through inactivation of chemical mediators. Therefore, estimation of ECP and simultaneously arylsufatase B in nasal secretion and the sera from patients with nasal allergy may clarify the dynamics of clinico-pathological state, especially in the late phase of allergic reaction in each patients. ECP concentrations in the nasal secretions from 22 patients and in the sera from 12 patients with nasal allergy were measured by RIA method. The activities of arylsulfatase B in the nasal secretions and the sera were also estimated in the same specimens as ECP by measuring its hydrolytic activity using p-nitro cathecol sulfate as a substrate. The results obtained were as follows ;<br> 1) There was a significant correlation between ECP concentrations in the nasal secretions and the severities of clinical symptoms, especially the degree of nasal obstruction. ECP concentrations also significantly correlated to the score of eosinophilic leucocytes in the nasal smears. <br>2) The serum ECP concentrations significantly correlated to the number of eosinophilic leucocytes in the peripheral blood, and also showed slight tendency of correlation to the severity of clinical symptoms. <br>3) Arylsufatase B activities in the nasal secretions significantly correlated to the score of eosinophilic leucocytes in the nasal smears. There was a significant correlation between ECP concentration and arylsulfatase B activities in the nasal secretions. However, there was no correlation between arylsufatase B activities in nasal secretions and clinical symptoms. <br>From these results, it was suggested that estimation of ECP in nasal secretion would be available in evaluation of the severity of nasal allergy in relevance to the infiltration of eosinophilic leucocytes. And it was also suggested that since ECP and arylsulfatase B might be released from eosinophilic leucocytes with non-independent manner, the role of arylsulfatase B in down regulation of the allergic reaction was not significant.
収録刊行物
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- 日本耳鼻咽喉科学会会報
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日本耳鼻咽喉科学会会報 94 (6), 786-793, 1991
一般社団法人 日本耳鼻咽喉科頭頸部外科学会