Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors

DOI Web Site 被引用文献94件
  • Anne Kallioniemi
    Division of Molecular Cytometry, Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94143.
  • Olli-P. Kallioniemi
    Division of Molecular Cytometry, Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94143.
  • Damir Sudar
    Division of Molecular Cytometry, Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94143.
  • Denis Rutovitz
    Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, United Kingdom.
  • Joe W. Gray
    Division of Molecular Cytometry, Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94143.
  • Fred Waldman
    Division of Molecular Cytometry, Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94143.
  • Dan Pinkel
    Division of Molecular Cytometry, Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94143.

抄録

<jats:p>Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.</jats:p>

収録刊行物

  • Science

    Science 258 (5083), 818-821, 1992-10-30

    American Association for the Advancement of Science (AAAS)

被引用文献 (94)*注記

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