Expression cloning of a human corticotropin-releasing-factor receptor.

DOI Web Site 被引用文献17件
  • R Chen
    Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, CA 92037.
  • K A Lewis
    Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, CA 92037.
  • M H Perrin
    Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, CA 92037.
  • W W Vale
    Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, CA 92037.

抄録

<jats:p>Corticotropin-releasing factor (CRF) is the principal neuroregulator of the hypothalamic-pituitary-adrenocortical axis and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. We report here the cloning of a cDNA coding for a CRF receptor from a human corticotropic tumor library. The cloned cDNA encodes a 415-amino acid protein comprising seven putative membrane-spanning domains and is structurally related to the calcitonin/vasoactive intestinal peptide/growth hormone-releasing hormone subfamily of G protein-coupled receptors. The receptor expressed in COS cells binds rat/human CRF with high affinity (Kd = 3.3 +/- 0.45 nM) and specificity and is functionally coupled to adenylate cyclase. The CRF antagonist alpha-helCRF-(9-41) inhibits the CRF-stimulated increase in intracellular cAMP. Northern blot analysis reveals that the CRF receptor is expressed in the rat pituitary and brain as well as in the mouse AtT20 corticotropic cells. We also describe an alternatively spliced form of the receptor which includes an insert of 29 amino acids in the first intracellular loop.</jats:p>

収録刊行物

被引用文献 (17)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ