Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
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- S Inoue
- Department of Biochemistry, Saitama Medical School, Japan.
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- A Orimo
- Department of Biochemistry, Saitama Medical School, Japan.
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- T Hosoi
- Department of Biochemistry, Saitama Medical School, Japan.
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- S Kondo
- Department of Biochemistry, Saitama Medical School, Japan.
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- H Toyoshima
- Department of Biochemistry, Saitama Medical School, Japan.
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- T Kondo
- Department of Biochemistry, Saitama Medical School, Japan.
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- A Ikegami
- Department of Biochemistry, Saitama Medical School, Japan.
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- Y Ouchi
- Department of Biochemistry, Saitama Medical School, Japan.
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- H Orimo
- Department of Biochemistry, Saitama Medical School, Japan.
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- M Muramatsu
- Department of Biochemistry, Saitama Medical School, Japan.
抄録
<jats:p>Estrogen receptor (ER)-binding fragments were isolated from human genomic DNA by using a recombinant ER protein. Using one of these fragments as a probe, we have identified an estrogen-responsive gene that encodes a putative zinc finger protein. It has a RING finger motif present in a family of apparent DNA-binding proteins and is designated estrogen-responsive finger protein (efp). efp cDNA contains a consensus estrogen-responsive element at the 3' untranslated region that can act as a downstream estrogen-dependent enhancer. Moreover, efp is regulated by estrogen as demonstrated at both the mRNA and the protein level in ER-positive cells derived from mammary gland. These data suggest that efp may represent an estrogen-responsive transcription factor that mediates phenotypic expression of the diverse estrogen action. Thus, the genomic binding-site cloning may be applicable for isolation of the target genes of other transcription factors.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 90 (23), 11117-11121, 1993-12
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1361418520298360576
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- NII論文ID
- 80007374293
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- ISSN
- 10916490
- 00278424
- http://id.crossref.org/issn/00278424
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