ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons.

DOI PDF 被引用文献43件
  • S Tsukita
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan.
  • K Oishi
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan.
  • N Sato
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan.
  • J Sagara
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan.
  • A Kawai
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan.

抄録

<jats:p>The ERM family members, ezrin, radixin, and moesin, localizing just beneath the plasma membranes, are thought to be involved in the actin filament/plasma membrane association. To identify the integral membrane protein directly associated with ERM family members, we performed immunoprecipitation studies using antimoesin mAb and cultured baby hamster kidney (BHK) cells metabolically labeled with [35S]methionine or surface-labeled with biotin. The results indicated that moesin is directly associated with a 140-kD integral membrane protein. Using BHK cells as antigens, we obtained a mAb that recognized the 140-kD membrane protein. We next cloned a cDNA encoding the 140-kD membrane protein and identified it as CD44, a broadly distributed cell surface glycoprotein. Immunoprecipitation with various anti-CD44 mAbs showed that ezrin and radixin, as well as moesin, are associated with CD44, not only in BHK cells, but also in mouse L fibroblasts. Furthermore, immunofluorescence microscopy revealed that in both BHK and L cells, the Triton X-100-insoluble CD44 is precisely colocalized with ERM family members. We concluded that ERM family members work as molecular linkers between the cytoplasmic domain of CD44 and actin-based cytoskeletons.</jats:p>

収録刊行物

被引用文献 (43)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ