Dedikaryotization of the Shitake mushroom, Lentinula edodes by the protoplast regeneration method.
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- FUKUMASA-NAKAI YUKITAKA
- The Tottori Mycological Institute
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- MATSUMOTO TERUYUKI
- The Tottori Mycological Institute
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- KOMATSU MITSUO
- The Tottori Mycological Institute
抄録
The present study was carried out to investigate the applicability and usefulness of the protoplast isolation and regeneration method as a new technique for artificially dedikaryotizing Lentinula edodes dikaryons. When protoplasts derived from dikaryons were incubated in a regeneration agar medium at 25°C, about 11% of them individually started regeneration into hyphae within 3 days and formed visible colonies, varying in size, after 7 days of incubation. By isolating preferentially smaller colonies out of these visible colonies, it was found that neohaplonts could be obtained at the high frequencies of 40-92% in every dikaryon sampled and that the two-component nuclear types appeared. These neohaplonts showed considerable variation of mycelial growth rates. However, robust neohaplonts exhibited no apparent change in biological properties such as colony morphology, and electophoretic zymograms of esterase and malate dehydrogenase, suggesting that they might retain the original genetic traits. From these results, it is concluded that the protoplast regeneration method for dedikaryotizing L. edodes dikaryons is more useful than previous methods such as the physical procedure and chemical treatment.
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 40 (6), 551-562, 1994
公益財団法人 応用微生物学・分子細胞生物学研究奨励会
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詳細情報 詳細情報について
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- CRID
- 1390001204144122624
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- NII論文ID
- 130003399793
- 80008110021
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- COI
- 1:CAS:528:DyaK2MXks12hsrY%3D
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- ISSN
- 13498037
- 00221260
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可