D-serine, an endogenous synaptic modulator: localization to astrocytes and glutamate-stimulated release.

• M J Schell

  Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

• M E Molliver

  Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

• S H Snyder

  Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Abstract

Using an antibody highly specific for D-serine conjugated to glutaraldehyde, we have localized endogenous D-serine in rat brain. Highest levels of D-serine immunoreactivity occur in the gray matter of the cerebral cortex, hippocampus, anterior olfactory nucleus, olfactory tubercle, and amygdala. Localizations of D-serine immunoreactivity correlate closely with those of D-serine binding to the glycine modulatory site of the N-methyl-D-aspartate (NMDA) receptor as visualized by autoradiography and are inversely correlated to the presence of D-amino acid oxidase. D-Serine is enriched in process-bearing glial cells in neuropil with the morphology of protoplasmic astrocytes. In glial cultures of rat cerebral cortex, D-serine is enriched in type 2 astrocytes. The release of D-serine from these cultures is stimulated by agonists of non-NMDA glutamate receptors, suggesting a mechanism by which astrocyte-derived D-serine could modulate neurotransmission. D-Serine appears to be the endogenous ligand for the glycine site of NMDA receptors.

Journal

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 92 (9), 3948-3952, 1995-04-25

  Proceedings of the National Academy of Sciences

Keywords

• Multidisciplinary

Details

• CRID: 1363951794392356608
• NII Article ID: 80008294279
• DOI: 10.1073/pnas.92.9.3948
• ISSN: 10916490
• ISSN: 00278424
• ISSN: http://id.crossref.org/issn/00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.92.9.3948
• Data Source:
  • Crossref
  • CiNii Articles