Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell

Masatake Osawa, Ken-ichi Hanada, Hirofumi Hamada, Hiromitsu Nakauchi

doi:10.1126/science.273.5272.242

Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell

DOI Web Site 被引用文献188件

Masatake Osawa

M. Osawa and H. Nakauchi, Department of Immunology, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba Science-City, Ibaraki 305, Japan.
Ken-ichi Hanada

K.-i. Hanada and H. Hamada, Department of Molecular Biotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo 170, Japan.
Hirofumi Hamada

K.-i. Hanada and H. Hamada, Department of Molecular Biotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo 170, Japan.
Hiromitsu Nakauchi

M. Osawa and H. Nakauchi, Department of Immunology, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba Science-City, Ibaraki 305, Japan.

抄録

<jats:p> Hematopoietic stem cells (HSCs) supply all blood cells throughout life by making use of their self-renewal and multilineage differentiation capabilities. A monoclonal antibody raised to the mouse homolog of CD34 (mCD34) was used to purify mouse HSCs to near homogeneity. Unlike in humans, primitive adult mouse bone marrow HSCs were detected in the mCD34 low to negative fraction. Injection of a single mCD34 <jats:sup>lo/−</jats:sup> , c-Kit <jats:sup>+</jats:sup> , Sca-1 <jats:sup>+</jats:sup> , lineage markers negative (Lin <jats:sup>−</jats:sup> ) cell resulted in long-term reconstitution of the lymphohematopoietic system in 21 percent of recipients. Thus, the purified HSC population should enable analysis of the self-renewal and multilineage differentiation of individual HSCs. </jats:p>