Early embryonic lethality caused by targeted disruption of the mouse selenocysteine tRNA gene ( <i>Trsp</i> )
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- Michael R. Bösl
- Banyu Tsukuba Research Institute (Merck), Tsukuba 300-26, Japan; and Laboratory of Biomedical Genetics, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, 113, Japan
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- Kazuaki Takaku
- Banyu Tsukuba Research Institute (Merck), Tsukuba 300-26, Japan; and Laboratory of Biomedical Genetics, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, 113, Japan
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- Masanobu Oshima
- Banyu Tsukuba Research Institute (Merck), Tsukuba 300-26, Japan; and Laboratory of Biomedical Genetics, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, 113, Japan
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- Susumu Nishimura
- Banyu Tsukuba Research Institute (Merck), Tsukuba 300-26, Japan; and Laboratory of Biomedical Genetics, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, 113, Japan
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- Makoto M. Taketo
- Banyu Tsukuba Research Institute (Merck), Tsukuba 300-26, Japan; and Laboratory of Biomedical Genetics, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, 113, Japan
抄録
<jats:p> Selenoprotein biosynthesis is mediated by tRNA <jats:sup>Sec</jats:sup> , which inserts selenocysteine at UGA codons in a complex, context-specific manner. This opal suppressor serves in the conversion of serine to selenocysteine as well. The mouse tRNA <jats:sup>Sec</jats:sup> gene ( <jats:italic>Trsp</jats:italic> ) maps to a proximal segment of chromosome 7. We constructed mice carrying a targeted deletion of the <jats:italic>Trsp</jats:italic> gene. The heterozygous mutants were viable, fertile, and appeared normal. Although the level of tRNA <jats:sup>Sec</jats:sup> was reduced to about 50%–80% of the wild type in most organs, one of the selenoproteins, glutathione peroxidase, remained unaffected in the levels of its mRNA, protein, and enzyme activity, indicating that the haploid amount of tRNA <jats:sup>Sec</jats:sup> is not limiting in its biosynthesis. In contrast, the homozygous mutants died shortly after implantation, and the embryos were resorbed before 6.5 days post coitum. When the preimplantation embryos were placed in culture, however, the trophoectoderm cells showed outgrowths and the inner cell mass cells of the homozygous embryos were able to proliferate. These results indicate that <jats:italic>Trsp</jats:italic> expression is essential for early development of the embryo, and its lack causes peri-implantation lethality. However, the lethality does not appear to be due to a cell-autonomous function of tRNA <jats:sup>Sec</jats:sup> . </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 94 (11), 5531-5534, 1997-05-27
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1363107370261675904
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- NII論文ID
- 80009716096
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- ISSN
- 10916490
- 00278424
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