Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale

DOI Web Site 被引用文献116件
  • Joseph L. DeRisi
    Department of Biochemistry, Stanford University School of Medicine, Howard Hughes Medical Institute, Stanford, CA 94305–5428, USA.
  • Vishwanath R. Iyer
    Department of Biochemistry, Stanford University School of Medicine, Howard Hughes Medical Institute, Stanford, CA 94305–5428, USA.
  • Patrick O. Brown
    Department of Biochemistry, Stanford University School of Medicine, Howard Hughes Medical Institute, Stanford, CA 94305–5428, USA.

抄録

<jats:p> DNA microarrays containing virtually every gene of <jats:italic>Saccharomyces cerevisiae</jats:italic> were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration. The expression profiles observed for genes with known metabolic functions pointed to features of the metabolic reprogramming that occur during the diauxic shift, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions. The same DNA microarrays were also used to identify genes whose expression was affected by deletion of the transcriptional co-repressor <jats:italic>TUP1</jats:italic> or overexpression of the transcriptional activator <jats:italic>YAP1</jats:italic> . These results demonstrate the feasibility and utility of this approach to genomewide exploration of gene expression patterns. </jats:p>

収録刊行物

  • Science

    Science 278 (5338), 680-686, 1997-10-24

    American Association for the Advancement of Science (AAAS)

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