Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronal activity

  • Nicola R. Sibson
    Departments of Molecular Biophysics and Biochemistry, Psychiatry, Diagnostic Radiology and Neurology, Yale University School of Medicine, New Haven, CT 06520
  • Ajay Dhankhar
    Departments of Molecular Biophysics and Biochemistry, Psychiatry, Diagnostic Radiology and Neurology, Yale University School of Medicine, New Haven, CT 06520
  • Graeme F. Mason
    Departments of Molecular Biophysics and Biochemistry, Psychiatry, Diagnostic Radiology and Neurology, Yale University School of Medicine, New Haven, CT 06520
  • Douglas L. Rothman
    Departments of Molecular Biophysics and Biochemistry, Psychiatry, Diagnostic Radiology and Neurology, Yale University School of Medicine, New Haven, CT 06520
  • Kevin L. Behar
    Departments of Molecular Biophysics and Biochemistry, Psychiatry, Diagnostic Radiology and Neurology, Yale University School of Medicine, New Haven, CT 06520
  • Robert G. Shulman
    Departments of Molecular Biophysics and Biochemistry, Psychiatry, Diagnostic Radiology and Neurology, Yale University School of Medicine, New Haven, CT 06520

抄録

<jats:p> To determine the relationship between cerebral Glc metabolism and glutamatergic neuronal function, we used <jats:sup>13</jats:sup> C NMR spectroscopy to measure, simultaneously, the rates of the tricarboxylic acid cycle and Gln synthesis in the rat cortex <jats:italic>in vivo</jats:italic> . From these measurements, we calculated the rates of oxidative Glc metabolism and glutamate–neurotransmitter cycling between neurons and astrocytes (a quantitative measure of glutamatergic neuronal activity). By measuring the rates of the tricarboxylic acid cycle and Gln synthesis over a range of synaptic activity, we have determined the stoichiometry between oxidative Glc metabolism and glutamate–neurotransmitter cycling in the cortex to be close to 1:1. This finding indicates that the majority of cortical energy production supports functional (synaptic) glutamatergic neuronal activity. Another implication of this result is that brain activation studies, which map cortical oxidative Glc metabolism, provide a quantitative measure of synaptic glutamate release. </jats:p>

収録刊行物

被引用文献 (13)*注記

もっと見る

キーワード

詳細情報

問題の指摘

ページトップへ