The Heat-Shock Element Is a Functional Component of the Arabidopsis <i>APX1</i> Gene Promoter

DOI PDF 被引用文献10件
  • Sergei Storozhenko
    Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie (S.S., P.D.P., M.V.M., S.K.)
  • Pascal De Pauw
    Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie (S.S., P.D.P., M.V.M., S.K.)
  • Marc Van Montagu
    Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie (S.S., P.D.P., M.V.M., S.K.)
  • Dirk Inzé
    Laboratoire Associé de l'Institut National de la Recherche Agronomique (France) (D.I.), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
  • Sergei Kushnir
    Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie (S.S., P.D.P., M.V.M., S.K.)

抄録

<jats:title>Abstract</jats:title> <jats:p>Ascorbate peroxidases are important enzymes that detoxify hydrogen peroxide within the cytosol and chloroplasts of plant cells. To better understand their role in oxidative stress tolerance, the transcriptional regulation of theapx1 gene from Arabidopsis was studied. The apx1 gene was expressed in all tested organs of Arabidopsis; mRNA levels were low in roots, leaves, and stems and high in flowers. Steady-state mRNA levels in leaves or cell suspensions increased after treatment with methyl viologen, ethephon, high temperature, and illumination of etiolated seedlings. A putative heat-shock cis element found in the apx1promoter was shown to be recognized by the tomato (Lycopersicon esculentum) heat-shock factor in vitro and to be responsible for the in vivo heat-shock induction of the gene. The heat-shockcis element also contributed partially to the induction of the gene by oxidative stress. By using in vivo dimethyl sulfate footprinting, we showed that proteins interacted with a G/C-rich element found in the apx1 promoter.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 118 (3), 1005-1014, 1998-11-01

    Oxford University Press (OUP)

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