A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants

• Andrew J. Hamilton

  Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.

• David C. Baulcombe

  Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.

抄録

Posttranscriptional gene silencing (PTGS) is a nucleotide sequence–specific defense mechanism that can target both cellular and viral mRNAs. Here, three types of transgene-induced PTGS and one example of virus-induced PTGS were analyzed in plants. In each case, antisense RNA complementary to the targeted mRNA was detected. These RNA molecules were of a uniform length, estimated at 25 nucleotides, and their accumulation required either transgene sense transcription or RNA virus replication. Thus, the 25-nucleotide antisense RNA is likely synthesized from an RNA template and may represent the specificity determinant of PTGS.

収録刊行物

• Science

  Science 286 (5441), 950-952, 1999-10-29

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361699995488568192
• NII論文ID: 80010740141
• DOI: 10.1126/science.286.5441.950
• ISSN: 10959203
• ISSN: 00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.286.5441.950
• データソース種別:
  • Crossref
  • CiNii Articles