An abscisic acid-induced protein kinase, PKABA1, mediates abscisic acid-suppressed gene expression in barley aleurone layers

• Aurelio Gómez-Cadenas

  Department of Biology, Washington University, St. Louis, MO 63130; and U.S. Department of Agriculture–Agriculture Research Service, Washington State University, Pullman, WA 99164-6420

• Steven D. Verhey

  Department of Biology, Washington University, St. Louis, MO 63130; and U.S. Department of Agriculture–Agriculture Research Service, Washington State University, Pullman, WA 99164-6420

• Lynn D. Holappa

  Department of Biology, Washington University, St. Louis, MO 63130; and U.S. Department of Agriculture–Agriculture Research Service, Washington State University, Pullman, WA 99164-6420

• Qingxi Shen

  Department of Biology, Washington University, St. Louis, MO 63130; and U.S. Department of Agriculture–Agriculture Research Service, Washington State University, Pullman, WA 99164-6420

• Tuan-Hua David Ho

  Department of Biology, Washington University, St. Louis, MO 63130; and U.S. Department of Agriculture–Agriculture Research Service, Washington State University, Pullman, WA 99164-6420

• M. K. Walker-Simmons

  Department of Biology, Washington University, St. Louis, MO 63130; and U.S. Department of Agriculture–Agriculture Research Service, Washington State University, Pullman, WA 99164-6420

抄録

The phytohormone abscisic acid (ABA) induces genes-encoding proteins involved in desiccation tolerance and dormancy in seeds, but ABA also suppresses gibberellin (GA)-responsive genes encoding hydrolytic enzymes essential for postgermination growth. A unique serine/threonine protein kinase, PKABA1 mRNA, up-regulated by ABA in seeds, has been identified. In this report, the effect of PKABA1 on the signal transduction pathway mediating ABA induction and suppression of genes has been determined in aleurone layers of barley seeds. Two groups of gene constructs were introduced to barley aleurone layers by using particle bombardment: the reporter constructs containing the coding sequence of β-glucuronidase gene linked to hormone-responsive promoters and the effector constructs containing the coding region of protein kinases linked to a constitutive promoter. Constitutive expression of PKABA1 drastically suppressed expression of low- and high-pI α-amylase and protease genes induced by GA. However, the presence of PKABA1 had only a small effect on the ABA induction of a gene encoding a late embryogenesis abundant protein, HVA1. Our results indicate that PKABA1 acts as a key intermediate in the signal transduction pathway leading to the suppression of GA-inducible gene expression in cereal aleurone layers.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 96 (4), 1767-1772, 1999-02-16

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1362544421078404608
• NII論文ID: 80010968443
• DOI: 10.1073/pnas.96.4.1767
• ISSN: 10916490
• ISSN: 00278424
• ISSN: http://id.crossref.org/issn/0015749X
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.96.4.1767
• データソース種別:
  • Crossref
  • CiNii Articles