Purification and Cloning of Aggrecanase-1: A Member of the ADAMTS Family of Proteins

• M. D. Tortorella

  Department of Inflammatory Diseases Research,

• T. C. Burn

  Department of Applied Biotechnology,

• M. A. Pratta

  Department of Inflammatory Diseases Research,

• I. Abbaszade

  Department of Applied Biotechnology,

• J. M. Hollis

  Department of Applied Biotechnology,

• R. Liu

  Department of Inflammatory Diseases Research,

• S. A. Rosenfeld

  Department of Applied Biotechnology,

• R. A. Copeland

  Department of Enzymology,

• C. P. Decicco

  Department of Chemical and Physical Sciences, DuPont Pharmaceuticals Company, Wilmington, DE 19880–0400, USA.

• R. Wynn

  Department of Applied Biotechnology,

• A. Rockwell

  Department of Chemical and Physical Sciences, DuPont Pharmaceuticals Company, Wilmington, DE 19880–0400, USA.

• F. Yang

  Department of Enzymology,

• J. L. Duke

  Department of Applied Biotechnology,

• K. Solomon

  Department of Inflammatory Diseases Research,

• H. George

  Department of Applied Biotechnology,

• R. Bruckner

  Department of Inflammatory Diseases Research,

• H. Nagase

  Department of Biochemistry and Molecular Biology, University of Kansas School of Medicine, Kansas City, KS 66160, USA.

• Y. Itoh

  Department of Biochemistry and Molecular Biology, University of Kansas School of Medicine, Kansas City, KS 66160, USA.

• D. M. Ellis

  Department of Applied Biotechnology,

• H. Ross

  Department of Applied Biotechnology,

• B. H. Wiswall

  Department of Applied Biotechnology,

• K. Murphy

  Department of Applied Biotechnology,

• M. C. Hillman

  Department of Applied Biotechnology,

• G. F. Hollis

  Department of Applied Biotechnology,

• R. C. Newton

  Department of Inflammatory Diseases Research,

• R. L. Magolda

  Department of Inflammatory Diseases Research,

• J. M. Trzaskos

  Department of Inflammatory Diseases Research,

• E. C. Arner

  Department of Inflammatory Diseases Research,

抄録

We purified, cloned, and expressed aggrecanase, a protease that is thought to be responsible for the degradation of cartilage aggrecan in arthritic diseases. Aggrecanase-1 [a disintegrin and metalloproteinase with thrombospondin motifs–4 (ADAMTS-4)] is a member of the ADAMTS protein family that cleaves aggrecan at the glutamic acid-373–alanine-374 bond. The identification of this protease provides a specific target for the development of therapeutics to prevent cartilage degradation in arthritis.

収録刊行物

• Science

  Science 284 (5420), 1664-1666, 1999-06-04

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1363107370293802368
• NII論文ID: 80011134048
• DOI: 10.1126/science.284.5420.1664
• ISSN: 10959203
• ISSN: 00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.284.5420.1664
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  • CiNii Articles