Abortive expansion of the cumulus and impaired fertility in mice lacking the prostaglandin E receptor subtype EP ₂

• Hiroko Hizaki

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Eri Segi

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Yukihiko Sugimoto

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Masaya Hirose

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Tomomi Saji

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Fumitaka Ushikubi

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Toshiyuki Matsuoka

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Yoichi Noda

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Takashi Tanaka

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Nobuaki Yoshida

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Shuh Narumiya

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

• Atsushi Ichikawa

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, and Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu 520-21, Japan; and Division of Molecular and Cellular Immunology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka 594-1101, Japan

抄録

Female mice lacking the gene encoding the prostaglandin (PG) E 2 receptor subtype EP 2 (EP 2 −/− ) become pregnant and deliver their pups at term, but with a much reduced litter size. A decrease in ovulation number and a much reduced fertilization rate were observed in EP 2 −/− females without difference of the uterus to support implantation of wild-type embryos. Treatment with gonadotropins induced EP 2 mRNA expression in the cumulus cells of ovarian follicles of wild-type mice. The immature cumuli oophori from wild-type mice expanded in vitro in response to both follicle-stimulating hormone and PGE 2 , but the response to PGE 2 was absent in those from EP 2 −/− mice. Cumulus expansion proceeded normally in preovulatory follicles but became abortive in a number of ovulated complexes in EP 2 −/− mice, indicating that EP 2 is involved in cumulus expansion in the oviduct in vivo . No difference in the fertilization rate between wild-type and EP 2 −/− mice was found in in vitro studies using cumulus-free oocytes. These results indicate that PGE 2 cooperates with gonadotropin to complete cumulus expansion for successful fertilization.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 96 (18), 10501-10506, 1999-08-31

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1364233270494555904
• NII論文ID: 80011327238
• DOI: 10.1073/pnas.96.18.10501
• ISSN: 10916490
• ISSN: 00278424
• ISSN: http://id.crossref.org/issn/00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.96.18.10501
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