Global Transposon Mutagenesis and a Minimal Mycoplasma Genome

DOI Web Site 被引用文献17件
  • Clyde A. Hutchison
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
  • Scott N. Peterson
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
  • Steven R. Gill
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
  • Robin T. Cline
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
  • Owen White
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
  • Claire M. Fraser
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
  • Hamilton O. Smith
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
  • J. Craig Venter
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.

抄録

<jats:p> <jats:italic>Mycoplasma genitalium</jats:italic> with 517 genes has the smallest gene complement of any independently replicating cell so far identified. Global transposon mutagenesis was used to identify nonessential genes in an effort to learn whether the naturally occurring gene complement is a true minimal genome under laboratory growth conditions. The positions of 2209 transposon insertions in the completely sequenced genomes of <jats:italic>M. genitalium</jats:italic> and its close relative <jats:italic>M. pneumoniae</jats:italic> were determined by sequencing across the junction of the transposon and the genomic DNA. These junctions defined 1354 distinct sites of insertion that were not lethal. The analysis suggests that 265 to 350 of the 480 protein-coding genes of <jats:italic>M. genitalium</jats:italic> are essential under laboratory growth conditions, including about 100 genes of unknown function. </jats:p>

収録刊行物

  • Science

    Science 286 (5447), 2165-2169, 1999-12-10

    American Association for the Advancement of Science (AAAS)

被引用文献 (17)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ