Requirement of the Inositol Trisphosphate Receptor for Activation of Store-Operated Ca ²⁺ Channels

• Hong-Tao Ma

  Department of Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA.

• Randen L. Patterson

  Department of Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA.

• Damian B. van

• Rossum

  Department of Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA.

• Lutz Birnbaumer

  Department of Anesthesiology, University of California, Los Angeles, CA 90095, USA.

• Katsuhiko Mikoshiba

  Department of Molecular Neurobiology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.

• Donald L. Gill

  Department of Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA.

抄録

The coupling mechanism between endoplasmic reticulum (ER) calcium ion (Ca 2+ ) stores and plasma membrane (PM) store-operated channels (SOCs) is crucial to Ca 2+ signaling but has eluded detection. SOCs may be functionally related to the TRP family of receptor-operated channels. Direct comparison of endogenous SOCs with stably expressed TRP3 channels in human embryonic kidney (HEK293) cells revealed that TRP3 channels differ in being store independent. However, condensed cortical F-actin prevented activation of both SOC and TRP3 channels, which suggests that ER-PM interactions underlie coupling of both channels. A cell-permeant inhibitor of inositol trisphosphate receptor (InsP 3 R) function, 2-aminoethoxydiphenyl borate, prevented both receptor-induced TRP3 activation and store-induced SOC activation. It is concluded that InsP 3 Rs mediate both SOC and TRP channel opening and that the InsP 3 R is essential for maintaining coupling between store emptying and physiological activation of SOCs.

収録刊行物

• Science

  Science 287 (5458), 1647-1651, 2000-03-03

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1362262946432214784
• NII論文ID: 80011670574
• DOI: 10.1126/science.287.5458.1647
• ISSN: 10959203
• ISSN: 00368075
• ISSN: http://id.crossref.org/issn/00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.287.5458.1647
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