A Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus with Efficient Gene Transfer and Expression

• Hai-Ou Li

  <!--label omitted: 1-->DNAVEC Research Inc., Tsukuba-shi, Ibaraki 305-0856,1 and

• Ya-Feng Zhu

  <!--label omitted: 1-->DNAVEC Research Inc., Tsukuba-shi, Ibaraki 305-0856,1 and

• Makoto Asakawa

  <!--label omitted: 1-->DNAVEC Research Inc., Tsukuba-shi, Ibaraki 305-0856,1 and

• Hidekazu Kuma

  <!--label omitted: 1-->DNAVEC Research Inc., Tsukuba-shi, Ibaraki 305-0856,1 and

• Takahiro Hirata

  <!--label omitted: 1-->DNAVEC Research Inc., Tsukuba-shi, Ibaraki 305-0856,1 and

• Yasuji Ueda

  <!--label omitted: 1-->DNAVEC Research Inc., Tsukuba-shi, Ibaraki 305-0856,1 and

• Yun-Sik Lee

  <!--label omitted: 1-->DNAVEC Research Inc., Tsukuba-shi, Ibaraki 305-0856,1 and

• Masayuki Fukumura

  <!--label omitted: 1-->DNAVEC Research Inc., Tsukuba-shi, Ibaraki 305-0856,1 and

• Akihiro Iida

  <!--label omitted: 1-->DNAVEC Research Inc., Tsukuba-shi, Ibaraki 305-0856,1 and

• Atsushi Kato

  <!--label omitted: 2-->Department of Viral Infection, Institute of Medical Sciences, University of Tokyo, Minato-ku, Tokyo 108-8639,2 Japan

• Yoshiyuki Nagai

  <!--label omitted: 2-->Department of Viral Infection, Institute of Medical Sciences, University of Tokyo, Minato-ku, Tokyo 108-8639,2 Japan

• Mamoru Hasegawa

  <!--label omitted: 1-->DNAVEC Research Inc., Tsukuba-shi, Ibaraki 305-0856,1 and

抄録

We have recovered a virion from defective cDNA of Sendai virus (SeV) that is capable of self-replication but incapable of transmissible-virion production. This virion delivers and expresses foreign genes in infected cells, and this is the first report of a gene expression vector derived from a defective viral genome of the Paramyxoviridae . First, functional ribonucleoprotein complexes (RNPs) were recovered from SeV cloned cDNA defective in the F (envelope fusion protein) gene, in the presence of plasmids expressing nucleocapsid protein and viral RNA polymerase. Then the RNPs were transfected to the cells inducibly expressing F protein. Virion-like particles thus obtained had a titer of 0.5 × 10 8 to 1.0 × 10 8 cell infectious units/ml and contained F-defective RNA genome. This defective vector amplified specifically in an F-expressing packaging cell line in a trypsin-dependent manner but did not spread to F-nonexpressing cells. This vector infected and expressed an enhanced green fluorescent protein reporter gene in various types of animal and human cells, including nondividing cells, with high efficiency. These results suggest that this vector has great potential for use in human gene therapy and vaccine delivery systems.

収録刊行物

• Journal of Virology

  Journal of Virology 74 (14), 6564-6569, 2000-07-15

  American Society for Microbiology

キーワード

• Virology
• Insect Science
• Immunology
• Microbiology

詳細情報

• CRID: 1363107370182957952
• NII論文ID: 80011739199
• DOI: 10.1128/jvi.74.14.6564-6569.2000
• ISSN: 10985514
• ISSN: 0022538X
• Web Site: https://journals.asm.org/doi/pdf/10.1128/JVI.74.14.6564-6569.2000
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