Cytochrome P450-Dependent Metabolism of Oxylipins in Tomato. Cloning and Expression of Allene Oxide Synthase and Fatty Acid Hydroperoxide Lyase

• Gregg A. Howe

  Department of Energy-Plant Research Laboratory (G.A.H., G.I.L., A.I., L.L., A.E.D.) and

• Gyu In Lee

  Department of Energy-Plant Research Laboratory (G.A.H., G.I.L., A.I., L.L., A.E.D.) and

• Aya Itoh

  Department of Energy-Plant Research Laboratory (G.A.H., G.I.L., A.I., L.L., A.E.D.) and

• Lei Li

  Department of Energy-Plant Research Laboratory (G.A.H., G.I.L., A.I., L.L., A.E.D.) and

• Amy E. DeRocher

  Department of Energy-Plant Research Laboratory (G.A.H., G.I.L., A.I., L.L., A.E.D.) and

抄録

Allene oxide synthase (AOS) and fatty acid hydroperoxide lyase (HPL) are plant-specific cytochrome P450s that commit fatty acid hydroperoxides to different branches of oxylipin metabolism. Here we report the cloning and characterization of AOS (LeAOS) and HPL (LeHPL) cDNAs from tomato (Lycopersicon esculentum). Functional expression of the cDNAs inEscherichia coli showed that LeAOS andLeHPL encode enzymes that metabolize 13- but not 9-hydroperoxide derivatives of C18 fatty acids. LeAOS was active against both 13S-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid (13-HPOT) and 13S-hydroperoxy-9(Z),11(E)-octadecadienoic acid, whereas LeHPL showed a strong preference for 13-HPOT. These results suggest a role for LeAOS andLeHPL in the metabolism of 13-HPOT to jasmonic acid and hexenal/traumatin, respectively. LeAOS expression was detected in all organs of the plant. In contrast, LeHPLexpression was predominant in leaves and flowers. Damage inflicted to leaves by chewing insect larvae led to an increase in the local and systemic expression of both genes, with LeAOS showing the strongest induction. Wound-induced expression ofLeAOS also occurred in the def-1 mutant that is deficient in octadecanoid-based signaling of defensive proteinase inhibitor genes. These results demonstrate that tomato uses genetically distinct signaling pathways for the regulation of different classes of wound responsive genes.

収録刊行物

• Plant Physiology

  Plant Physiology 123 (2), 711-724, 2000-06-01

  Oxford University Press (OUP)

キーワード

• Plant Science
• Genetics
• Physiology

詳細情報

• CRID: 1360292619695141888
• NII論文ID: 80011972871
• DOI: 10.1104/pp.123.2.711
• ISSN: 15322548
• ISSN: 00320889
• Web Site: http://academic.oup.com/plphys/article-pdf/123/2/711/38673476/plphys_v123_2_711.pdf
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