Label-free probing of the binding state of DNA by time-domain terahertz sensing

• M. Brucherseifer

  Institut für Halbleitertechnik II, RWTH Aachen, Sommerfeldstr. 24, D-52056 Aachen, Germany

• M. Nagel

  Institut für Halbleitertechnik II, RWTH Aachen, Sommerfeldstr. 24, D-52056 Aachen, Germany

• P. Haring Bolivar

  Institut für Halbleitertechnik II, RWTH Aachen, Sommerfeldstr. 24, D-52056 Aachen, Germany

• H. Kurz

  Institut für Halbleitertechnik II, RWTH Aachen, Sommerfeldstr. 24, D-52056 Aachen, Germany

• A. Bosserhoff

  Institut für Pathologie, RWTH Aachen, Pauwelsstraße 30, D-52074 Aachen, Germany

• R. Büttner

  Institut für Pathologie, RWTH Aachen, Pauwelsstraße 30, D-52074 Aachen, Germany

抄録

We present a promising approach for the label-free characterization of genetic material. Time-resolved terahertz (THz) transmission analysis of polynucleotides demonstrate a strong dependence of the complex refractive index on the binding state (hybridized/denatured) of deoxyribonucleic acid (DNA) molecules. By monitoring THz transients, one can thus infer the binding state of oligo- and polynucleotides, and hence identify polynucleotides by detecting the binding of unknown polynucleotide DNA sequences to known probe molecules. A broadband experimental demonstration in a free-space configuration, as well as a discussion of the potential application for next generation gene chips is presented.

収録刊行物

• Applied Physics Letters

  Applied Physics Letters 77 (24), 4049-4051, 2000-12-11

  AIP Publishing

キーワード

• Physics and Astronomy (miscellaneous)

詳細情報

• CRID: 1362825894067793024
• NII論文ID: 80012118918
• DOI: 10.1063/1.1332415
• ISSN: 10773118
• ISSN: 00036951
• Web Site: https://pubs.aip.org/aip/apl/article-pdf/77/24/4049/18554154/4049_1_online.pdf
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