Antibiotic Susceptibility Profiles of <i>Escherichia coli</i> Strains Lacking Multidrug Efflux Pump Genes

• Mark C. Sulavik

  <!--label omitted: 1-->Genome Therapeutics Corporation, Waltham, Massachusetts,1 and

• Chad Houseweart

  <!--label omitted: 1-->Genome Therapeutics Corporation, Waltham, Massachusetts,1 and

• Christina Cramer

  <!--label omitted: 2--> Schering-Plough Research Institute, Kenilworth, New Jersey2

• Nilofer Jiwani

  <!--label omitted: 1-->Genome Therapeutics Corporation, Waltham, Massachusetts,1 and

• Nicholas Murgolo

  <!--label omitted: 2--> Schering-Plough Research Institute, Kenilworth, New Jersey2

• Jonathan Greene

  <!--label omitted: 2--> Schering-Plough Research Institute, Kenilworth, New Jersey2

• Beth DiDomenico

  <!--label omitted: 2--> Schering-Plough Research Institute, Kenilworth, New Jersey2

• Karen Joy Shaw

  <!--label omitted: 2--> Schering-Plough Research Institute, Kenilworth, New Jersey2

• George H. Miller

  <!--label omitted: 2--> Schering-Plough Research Institute, Kenilworth, New Jersey2

• Roberta Hare

  <!--label omitted: 2--> Schering-Plough Research Institute, Kenilworth, New Jersey2

• George Shimer

  <!--label omitted: 1-->Genome Therapeutics Corporation, Waltham, Massachusetts,1 and

抄録

The contribution of seven known and nine predicted genes or operons associated with multidrug resistance to the susceptibility of Escherichia coli W3110 was assessed for 20 different classes of antimicrobial compounds that include antibiotics, antiseptics, detergents, and dyes. Strains were constructed with deletions for genes in the major facilitator superfamily, the resistance nodulation-cell division family, the small multidrug resistance family, the ATP-binding cassette family, and outer membrane factors. The agar dilution MICs of 35 compounds were determined for strains with deletions for multidrug resistance (MDR) pumps. Deletions in acrAB or tolC resulted in increased susceptibilities to the majority of compounds tested. The remaining MDR pump gene deletions resulted in increased susceptibilities to far fewer compounds. The results identify which MDR pumps contribute to intrinsic resistance under the conditions tested and supply practical information useful for designing sensitive assay strains for cell-based screening of antibacterial compounds.

収録刊行物

• Antimicrobial Agents and Chemotherapy

  Antimicrobial Agents and Chemotherapy 45 (4), 1126-1136, 2001-04

  American Society for Microbiology

キーワード

• Infectious Diseases
• Pharmacology (medical)
• Pharmacology

詳細情報

• CRID: 1363670319531664256
• NII論文ID: 80012247276
• DOI: 10.1128/aac.45.4.1126-1136.2001
• ISSN: 10986596
• ISSN: 00664804
• Web Site: https://journals.asm.org/doi/pdf/10.1128/AAC.45.4.1126-1136.2001
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