<i>In vitro</i> assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore

• Hiroko Hanzawa

  Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan; and Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan

• Katsuhiko Inomata

  Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan; and Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan

• Hideki Kinoshita

  Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan; and Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan

• Takashi Kakiuchi

  Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan; and Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan

• Krishanthi Padmarani Jayasundera

  Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan; and Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan

• Daisuke Sawamoto

  Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan; and Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan

• Atsuko Ohta

  Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan; and Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan

• Kenko Uchida

  Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan; and Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan

• Keishiro Wada

  Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan; and Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan

• Masaki Furuya

  Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan; and Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan

抄録

Phytochrome B (PhyB), one of the major photosensory chromoproteins in plants, mediates a variety of light-responsive developmental processes in a photoreversible manner. To analyze the structural requirements of the chromophore for the spectral properties of PhyB, we have designed and chemically synthesized 20 analogs of the linear tetrapyrrole (bilin) chromophore and reconstituted them with PhyB apoprotein (PHYB). The A-ring acts mainly as the anchor for ligation to PHYB, because the modification of the side chains at the C2 and C3 positions did not significantly influence the formation or difference spectra of adducts. In contrast, the side chains of the B- and C-rings are crucial to position the chromophore properly in the chromophore pocket of PHYB and for photoreversible spectral changes. The side-chain structure of the D-ring is required for the photoreversible spectral change of the adducts. When methyl and ethyl groups at the C17 and C18 positions are replaced with an n -propyl, n -pentyl, or n -octyl group, respectively, the photoreversible spectral change of the adducts depends on the length of the side chains. From these studies, we conclude that each pyrrole ring of the linear tetrapyrrole chromophore plays a different role in chromophore assembly and the photochromic properties of PhyB.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 98 (6), 3612-3617, 2001-03-13

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360292619443014656
• NII論文ID: 80012335263
• DOI: 10.1073/pnas.051629698
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.051629698
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