Role of the arginine-nitric oxide pathway in the regulation of vascular smooth muscle cell proliferation

DOI Web Site 被引用文献8件
  • Louis J. Ignarro
    Department of Molecular and Medical Pharmacology, Center for the Health Sciences, University of California School of Medicine, Los Angeles, CA 90095; Cardiovascular Research Institute and Department of Medical Physiology, Texas A&M University System Health Science Center, College Station, TX 77843; and NicOx S.A., 06906 Sophia Antipolis, France
  • Georgette M. Buga
    Department of Molecular and Medical Pharmacology, Center for the Health Sciences, University of California School of Medicine, Los Angeles, CA 90095; Cardiovascular Research Institute and Department of Medical Physiology, Texas A&M University System Health Science Center, College Station, TX 77843; and NicOx S.A., 06906 Sophia Antipolis, France
  • Liu Hua Wei
    Department of Molecular and Medical Pharmacology, Center for the Health Sciences, University of California School of Medicine, Los Angeles, CA 90095; Cardiovascular Research Institute and Department of Medical Physiology, Texas A&M University System Health Science Center, College Station, TX 77843; and NicOx S.A., 06906 Sophia Antipolis, France
  • Philip M. Bauer
    Department of Molecular and Medical Pharmacology, Center for the Health Sciences, University of California School of Medicine, Los Angeles, CA 90095; Cardiovascular Research Institute and Department of Medical Physiology, Texas A&M University System Health Science Center, College Station, TX 77843; and NicOx S.A., 06906 Sophia Antipolis, France
  • Guoyao Wu
    Department of Molecular and Medical Pharmacology, Center for the Health Sciences, University of California School of Medicine, Los Angeles, CA 90095; Cardiovascular Research Institute and Department of Medical Physiology, Texas A&M University System Health Science Center, College Station, TX 77843; and NicOx S.A., 06906 Sophia Antipolis, France
  • Piero del Soldato
    Department of Molecular and Medical Pharmacology, Center for the Health Sciences, University of California School of Medicine, Los Angeles, CA 90095; Cardiovascular Research Institute and Department of Medical Physiology, Texas A&M University System Health Science Center, College Station, TX 77843; and NicOx S.A., 06906 Sophia Antipolis, France

抄録

<jats:p> The objective of this study was to elucidate the mechanisms by which nitric oxide (NO) inhibits rat aortic smooth muscle cell (RASMC) proliferation. Two products of the arginine-NO pathway interfere with cell growth by distinct mechanisms. N <jats:sup>G</jats:sup> -hydroxyarginine and NO appear to interfere with cell proliferation by inhibiting arginase and ornithine decarboxylase (ODC), respectively. <jats:italic>S</jats:italic> -nitroso- <jats:italic>N</jats:italic> -acetylpenicillamine, ( <jats:italic>Z</jats:italic> )-1-[ <jats:italic>N</jats:italic> -(2-aminoethyl)- <jats:italic>N</jats:italic> -(2-aminoethyl)-amino]-diazen-1-ium-1,2-diolate, and a nitroaspirin derivative (NCX 4016), each of which is a NO donor agent, inhibited RASMC growth at concentrations of 1–3 μM by cGMP-independent mechanisms. The cytostatic action of the NO donor agents as well as α-difluoromethylornithine (DFMO), a known ODC inhibitor, was prevented by addition of putrescine but not ornithine. These observations suggested that NO, like DFMO, may directly inhibit ODC. Experiments with purified, recombinant mammalian ODC revealed that NO inhibits ODC possibly by S-nitrosylation of the active site cysteine in ODC. DFMO, as well as the NO donor agents, interfered with cellular polyamine (putrescine, spermidine, spermine) production. Conversely, increasing the expression and catalytic activity of arginase I in RASMC either by transfection of cells with the arginase I gene or by induction of arginase I mRNA with IL-4 resulted in increased urea and polyamine production as well as cell proliferation. Finally, coculture of rat aortic endothelial cells, which had been pretreated with lipopolysaccharide plus a cytokine mixture to induce NO synthase and promote NO production, caused NO-dependent inhibition of target RASMC proliferation. This study confirms the inhibitory role of the arginine-NO pathway in vascular smooth muscle proliferation and indicates that one mechanism of action of NO is cGMP-independent and attributed to its capacity to inhibit ODC. </jats:p>

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