Aluminum Inhibits the H+-ATPase Activity by Permanently Altering the Plasma Membrane Surface Potentials in Squash Roots
-
- Sung Ju Ahn
- Research Institute for Bioresources, Okayama University, Chuo 2–20–1, Kurashiki 710–0046, Japan (S.J.A., M.S., H.O., H.M.);
-
- Mayandi Sivaguru
- Research Institute for Bioresources, Okayama University, Chuo 2–20–1, Kurashiki 710–0046, Japan (S.J.A., M.S., H.O., H.M.);
-
- Hiroki Osawa
- Research Institute for Bioresources, Okayama University, Chuo 2–20–1, Kurashiki 710–0046, Japan (S.J.A., M.S., H.O., H.M.);
-
- Gap Chae Chung
- Department of Horticulture, Biotechnology Research Institute, College of Agriculture, Chonnam National University, Kwangju 500–757, Korea (S.J.A, G.C.C.); and
-
- Hideaki Matsumoto
- Research Institute for Bioresources, Okayama University, Chuo 2–20–1, Kurashiki 710–0046, Japan (S.J.A., M.S., H.O., H.M.);
抄録
<jats:title>Abstract</jats:title> <jats:p>Although aluminum (AL) toxicity has been widely studied in monocotyledonous crop plants, the mechanism of Al impact on economically important dicotyledonous plants is poorly understood. Here, we report the spatial pattern of Al-induced root growth inhibition, which is closely associated with inhibition of H+-ATPase activity coupled with decreased surface negativity of plasma membrane (PM) vesicles isolated from apical 5-mm root segments of squash (Cucurbita pepo L. cv Tetsukabuto) plants. High-sensitivity growth measurements indicated that the central elongation zone, located 2 to 4 mm from the tip, was preferentially inhibited where high Al accumulation was found. The highest positive shifts (depolarization) in zeta potential of the isolated PM vesicles from 0- to 5-mm regions of Al-treated roots were corresponded to pronounced inhibition of H+-ATPase activity. The depolarization of PM vesicles isolated from Al-treated roots in response to added Al in vitro was less than that of control roots, suggesting, particularly in the first 5-mm root apex, a tight Al binding to PM target sites or irreversible alteration of PM properties upon Al treatment to intact plants. In line with these data, immunolocalization of H+-ATPase revealed decreases in tissue-specific H+-ATPase in the epidermal and cortex cells (2–3 mm from tip) following Al treatments. Our report provides the first circumstantial evidence for a zone-specific depolarization of PM surface potential coupled with inhibition of H+-ATPase activity. These effects may indicate a direct Al interaction with H+-ATPase from the cytoplasmic side of the PM.</jats:p>
収録刊行物
-
- Plant Physiology
-
Plant Physiology 126 (4), 1381-1390, 2001-08-01
Oxford University Press (OUP)
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1360292620684475264
-
- NII論文ID
- 80012556697
-
- ISSN
- 15322548
- 00320889
-
- データソース種別
-
- Crossref
- CiNii Articles