A comprehensive two-hybrid analysis to explore the yeast protein interactome
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- Takashi Ito
- Division of Genome Biology, Cancer Research Institute, Kanazawa University, Kanazawa 920-0934, Japan; Human Genome Research Group, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan; INTEC Web and Genome Informatics Corporation, Tokyo 136-0075, Japan; and Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
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- Tomoko Chiba
- Division of Genome Biology, Cancer Research Institute, Kanazawa University, Kanazawa 920-0934, Japan; Human Genome Research Group, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan; INTEC Web and Genome Informatics Corporation, Tokyo 136-0075, Japan; and Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
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- Ritsuko Ozawa
- Division of Genome Biology, Cancer Research Institute, Kanazawa University, Kanazawa 920-0934, Japan; Human Genome Research Group, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan; INTEC Web and Genome Informatics Corporation, Tokyo 136-0075, Japan; and Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
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- Mikio Yoshida
- Division of Genome Biology, Cancer Research Institute, Kanazawa University, Kanazawa 920-0934, Japan; Human Genome Research Group, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan; INTEC Web and Genome Informatics Corporation, Tokyo 136-0075, Japan; and Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
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- Masahira Hattori
- Division of Genome Biology, Cancer Research Institute, Kanazawa University, Kanazawa 920-0934, Japan; Human Genome Research Group, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan; INTEC Web and Genome Informatics Corporation, Tokyo 136-0075, Japan; and Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
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- Yoshiyuki Sakaki
- Division of Genome Biology, Cancer Research Institute, Kanazawa University, Kanazawa 920-0934, Japan; Human Genome Research Group, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan; INTEC Web and Genome Informatics Corporation, Tokyo 136-0075, Japan; and Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
抄録
<jats:p> Protein–protein interactions play crucial roles in the execution of various biological functions. Accordingly, their comprehensive description would contribute considerably to the functional interpretation of fully sequenced genomes, which are flooded with novel genes of unpredictable functions. We previously developed a system to examine two-hybrid interactions in all possible combinations between the ≈6,000 proteins of the budding yeast <jats:italic>Saccharomyces cerevisiae</jats:italic> . Here we have completed the comprehensive analysis using this system to identify 4,549 two-hybrid interactions among 3,278 proteins. Unexpectedly, these data do not largely overlap with those obtained by the other project [Uetz, P., <jats:italic>et al.</jats:italic> (2000) <jats:italic>Nature (London)</jats:italic> 403, 623–627] and hence have substantially expanded our knowledge on the protein interaction space or interactome of the yeast. Cumulative connection of these binary interactions generates a single huge network linking the vast majority of the proteins. Bioinformatics-aided selection of biologically relevant interactions highlights various intriguing subnetworks. They include, for instance, the one that had successfully foreseen the involvement of a novel protein in spindle pole body function as well as the one that may uncover a hitherto unidentified multiprotein complex potentially participating in the process of vesicular transport. Our data would thus significantly expand and improve the protein interaction map for the exploration of genome functions that eventually leads to thorough understanding of the cell as a molecular system. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 98 (8), 4569-4574, 2001-03-13
Proceedings of the National Academy of Sciences