Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis

• Caryn E. Outten

  Department of Chemistry and

• and Thomas V. O'Halloran

  Department of Chemistry and

抄録

Intracellular zinc is thought to be available in a cytosolic pool of free or loosely bound Zn(II) ions in the micromolar to picomolar range. To test this, we determined the mechanism of zinc sensors that control metal uptake or export in Escherichia coli and calibrated their response against the thermodynamically defined free zinc concentration. Whereas the cellular zinc quota is millimolar, free Zn(II) concentrations that trigger transcription of zinc uptake or efflux machinery are femtomolar, or six orders of magnitude less than one atom per cell. This is not consistent with a cytosolic pool of free Zn(II) and suggests an extraordinary intracellular zinc-binding capacity. Thus, cells exert tight control over cytosolic metal concentrations, even for relatively low-toxicity metals such as zinc.

収録刊行物

• Science

  Science 292 (5526), 2488-2492, 2001-06-29

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360855569104583040
• NII論文ID: 80012703141
• DOI: 10.1126/science.1060331
• ISSN: 10959203
• ISSN: 00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.1060331
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