<i>Arabidopsis</i> nph1 and npl1: Blue light receptors that mediate both phototropism and chloroplast relocation
-
- Tatsuya Sakai
- RIKEN (The Institute of Physical and Chemical Research) Plant Science Center, Sakyo-ku, Kyoto 606-8502, Japan; Unit Process and Combined Circuit, PRESTO (Precursory Research for Embryonic Science and Technology), Japan Science and Technology Corporation, 1-8, Honcho 4-chome, Kawaguchi-city, Saitama 332-0012, Japan; Division of Biological Regulation and Photobiology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Department of Plant Biology, Carnegie Institution of Washington,...
-
- Takatoshi Kagawa
- RIKEN (The Institute of Physical and Chemical Research) Plant Science Center, Sakyo-ku, Kyoto 606-8502, Japan; Unit Process and Combined Circuit, PRESTO (Precursory Research for Embryonic Science and Technology), Japan Science and Technology Corporation, 1-8, Honcho 4-chome, Kawaguchi-city, Saitama 332-0012, Japan; Division of Biological Regulation and Photobiology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Department of Plant Biology, Carnegie Institution of Washington,...
-
- Masahiro Kasahara
- RIKEN (The Institute of Physical and Chemical Research) Plant Science Center, Sakyo-ku, Kyoto 606-8502, Japan; Unit Process and Combined Circuit, PRESTO (Precursory Research for Embryonic Science and Technology), Japan Science and Technology Corporation, 1-8, Honcho 4-chome, Kawaguchi-city, Saitama 332-0012, Japan; Division of Biological Regulation and Photobiology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Department of Plant Biology, Carnegie Institution of Washington,...
-
- Trevor E. Swartz
- RIKEN (The Institute of Physical and Chemical Research) Plant Science Center, Sakyo-ku, Kyoto 606-8502, Japan; Unit Process and Combined Circuit, PRESTO (Precursory Research for Embryonic Science and Technology), Japan Science and Technology Corporation, 1-8, Honcho 4-chome, Kawaguchi-city, Saitama 332-0012, Japan; Division of Biological Regulation and Photobiology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Department of Plant Biology, Carnegie Institution of Washington,...
-
- John M. Christie
- RIKEN (The Institute of Physical and Chemical Research) Plant Science Center, Sakyo-ku, Kyoto 606-8502, Japan; Unit Process and Combined Circuit, PRESTO (Precursory Research for Embryonic Science and Technology), Japan Science and Technology Corporation, 1-8, Honcho 4-chome, Kawaguchi-city, Saitama 332-0012, Japan; Division of Biological Regulation and Photobiology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Department of Plant Biology, Carnegie Institution of Washington,...
-
- Winslow R. Briggs
- RIKEN (The Institute of Physical and Chemical Research) Plant Science Center, Sakyo-ku, Kyoto 606-8502, Japan; Unit Process and Combined Circuit, PRESTO (Precursory Research for Embryonic Science and Technology), Japan Science and Technology Corporation, 1-8, Honcho 4-chome, Kawaguchi-city, Saitama 332-0012, Japan; Division of Biological Regulation and Photobiology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Department of Plant Biology, Carnegie Institution of Washington,...
-
- Masamitsu Wada
- RIKEN (The Institute of Physical and Chemical Research) Plant Science Center, Sakyo-ku, Kyoto 606-8502, Japan; Unit Process and Combined Circuit, PRESTO (Precursory Research for Embryonic Science and Technology), Japan Science and Technology Corporation, 1-8, Honcho 4-chome, Kawaguchi-city, Saitama 332-0012, Japan; Division of Biological Regulation and Photobiology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Department of Plant Biology, Carnegie Institution of Washington,...
-
- Kiyotaka Okada
- RIKEN (The Institute of Physical and Chemical Research) Plant Science Center, Sakyo-ku, Kyoto 606-8502, Japan; Unit Process and Combined Circuit, PRESTO (Precursory Research for Embryonic Science and Technology), Japan Science and Technology Corporation, 1-8, Honcho 4-chome, Kawaguchi-city, Saitama 332-0012, Japan; Division of Biological Regulation and Photobiology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Department of Plant Biology, Carnegie Institution of Washington,...
抄録
<jats:p> UV-A/blue light acts to regulate a number of physiological processes in higher plants. These include light-driven chloroplast movement and phototropism. The <jats:italic>NPH1</jats:italic> gene of <jats:italic>Arabidopsis</jats:italic> encodes an autophosphorylating protein kinase that functions as a photoreceptor for phototropism in response to low-intensity blue light. However, <jats:italic>nph1</jats:italic> mutants have been reported to exhibit normal phototropic curvature under high-intensity blue light, indicating the presence of an additional phototropic receptor. A likely candidate is the nph1 homologue, npl1, which has recently been shown to mediate the avoidance response of chloroplasts to high-intensity blue light in <jats:italic>Arabidopsis.</jats:italic> Here we demonstrate that npl1, like nph1, noncovalently binds the chromophore flavin mononucleotide (FMN) within two specialized PAS domains, termed LOV domains. Furthermore, when expressed in insect cells, npl1, like nph1, undergoes light-dependent autophosphorylation, indicating that npl1 also functions as a light receptor kinase. Consistent with this conclusion, we show that a <jats:italic>nph1npl1</jats:italic> double mutant exhibits an impaired phototropic response under both low- and high-intensity blue light. Hence, npl1 functions as a second phototropic receptor under high fluence rate conditions and is, in part, functionally redundant to nph1. We also demonstrate that both chloroplast accumulation in response to low-intensity light and chloroplast avoidance movement in response to high-intensity light are lacking in the <jats:italic>nph1npl1</jats:italic> double mutant. Our findings therefore indicate that nph1 and npl1 show partially overlapping functions in two different responses, phototropism and chloroplast relocation, in a fluence rate-dependent manner. </jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 98 (12), 6969-6974, 2001-05-22
Proceedings of the National Academy of Sciences
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1363107368578625152
-
- NII論文ID
- 80012870849
-
- ISSN
- 10916490
- 00278424
- http://id.crossref.org/issn/00278424
-
- データソース種別
-
- Crossref
- CiNii Articles