An <i>mt</i>⁺ gamete-specific nuclease that targets <i>mt</i>⁻ chloroplasts during sexual reproduction in <i>C. reinhardtii</i>

• Yoshiki Nishimura

• Osami Misumi

• Ko Kato

• Noriko Inada

• Tetsuya Higashiyama

• Yu Momoyama

• Tsuneyoshi Kuroiwa

抄録

Although the active digestion of mating-type minus ( mt − ) chloroplast DNA (cpDNA) in young zygotes is considered to be the basis for the uniparental inheritance of cpDNA in Chlamydomonas reinhardtii , little is known about the underlying molecular mechanism. One model of active digestion proposes that nucleases are either synthesized or activated to digest mt − cpDNA. We used a native-PAGE/in gelo assay to investigate nuclease activities in chloroplasts from young zygotes, and identified a novel Ca 2+ -dependent nuclease activity. The timing of activation (∼60–90 min after mating) and the localization of the nuclease activity (in mt − chloroplasts) coincided with the active digestion of mt − cpDNA. Furthermore, the activity of the nuclease was coregulated with the maturation of mating-type plus ( mt + ) gametes, which would enable the efficient digestion of mt − cpDNA. Based on these observations, we propose that the nuclease (designated as M t + -specific DN ase, MDN) is a developmentally controlled nuclease that is activated in mt + gametes and participates in the destruction of mt − cpDNA in young zygotes, thereby ensuring uniparental inheritance of chloroplast traits.

収録刊行物

• Genes & Development

  Genes & Development 16 (9), 1116-1128, 2002-05-01

  Cold Spring Harbor Laboratory

キーワード

• Developmental Biology
• Genetics

詳細情報

• CRID: 1361137044781052160
• NII論文ID: 80015436597
• NII書誌ID: AA10668692
• DOI: 10.1101/gad.979902
• ISSN: 15495477
• ISSN: 08909369
• Web Site: https://syndication.highwire.org/content/doi/10.1101/gad.979902
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