Generation and in Vitro Differentiation of a Spermatogonial Cell Line

DOI Web Site 被引用文献18件
  • Li-Xin Feng
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA.
  • Yali Chen
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA.
  • Luis Dettin
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA.
  • Renee A. Reijo Pera
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, CA 94143, USA.
  • John C. Herr
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
  • Erwin Goldberg
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Chicago, IL 60208, USA.
  • Martin Dym
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA.

抄録

<jats:p>Spermatogenesis is the process by which spermatogonial stem cells divide and differentiate to produce sperm. In vitro sperm production has been difficult to achieve because of the lack of a culture system to maintain viable spermatogonia for long periods of time. Here we report the in vitro generation of spermatocytes and spermatids from telomerase-immortalized mouse type A spermatogonial cells in the presence of stem cell factor. This differentiation can occur in the absence of supportive cells. The immortalized spermatogonial cell line may serve as a powerful tool in elucidating the molecular mechanisms of spermatogenesis. Furthermore, through genomic modification and transplantation techniques, this male germ cell line may be used to generate transgenic mice and to develop germ cell gene therapy.</jats:p>

収録刊行物

  • Science

    Science 297 (5580), 392-395, 2002-07-19

    American Association for the Advancement of Science (AAAS)

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