Whole-Genome Shotgun Assembly and Analysis of the Genome of <i>Fugu rubripes</i>

• Samuel Aparicio

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Jarrod Chapman

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Elia Stupka

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Nik Putnam

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Jer-ming Chia

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Paramvir Dehal

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Alan Christoffels

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Sam Rash

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Shawn Hoon

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Arian Smit

  Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.

• Maarten D. Sollewijn Gelpke

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Jared Roach

  Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.

• Tania Oh

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Isaac Y. Ho

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Marie Wong

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Chris Detter

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Frans Verhoef

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Paul Predki

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Alice Tay

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Susan Lucas

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Paul Richardson

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Sarah F. Smith

  MRC UK HGMP Resource Centre, Hinxton, Cambridge CB10 1SB, UK.

• Melody S. Clark

  MRC UK HGMP Resource Centre, Hinxton, Cambridge CB10 1SB, UK.

• Yvonne J. K. Edwards

  MRC UK HGMP Resource Centre, Hinxton, Cambridge CB10 1SB, UK.

• Norman Doggett

  Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

• Andrey Zharkikh

  Myriad Genetics Inc., 320 Wakara Way, Salt Lake City, UT 84108, USA.

• Sean V. Tavtigian

  Myriad Genetics Inc., 320 Wakara Way, Salt Lake City, UT 84108, USA.

• Dmitry Pruss

  Myriad Genetics Inc., 320 Wakara Way, Salt Lake City, UT 84108, USA.

• Mary Barnstead

  Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.

• Cheryl Evans

  Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.

• Holly Baden

  Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.

• Justin Powell

  Paradigm Therapeutics Ltd., Physiological Laboratory, Cambridge CB2 3EG, UK.

• Gustavo Glusman

  Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.

• Lee Rowen

  Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.

• Leroy Hood

  Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.

• Y. H. Tan

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Greg Elgar

  MRC UK HGMP Resource Centre, Hinxton, Cambridge CB10 1SB, UK.

• Trevor Hawkins

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Byrappa Venkatesh

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

• Daniel Rokhsar

  U.S. DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.

• Sydney Brenner

  Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609.

抄録

The compact genome of Fugu rubripes has been sequenced to over 95% coverage, and more than 80% of the assembly is in multigene-sized scaffolds. In this 365-megabase vertebrate genome, repetitive DNA accounts for less than one-sixth of the sequence, and gene loci occupy about one-third of the genome. As with the human genome, gene loci are not evenly distributed, but are clustered into sparse and dense regions. Some “giant” genes were observed that had average coding sequence sizes but were spread over genomic lengths significantly larger than those of their human orthologs. Although three-quarters of predicted human proteins have a strong match to Fugu , approximately a quarter of the human proteins had highly diverged from or had no pufferfish homologs, highlighting the extent of protein evolution in the 450 million years since teleosts and mammals diverged. Conserved linkages between Fugu and human genes indicate the preservation of chromosomal segments from the common vertebrate ancestor, but with considerable scrambling of gene order.

収録刊行物

• Science

  Science 297 (5585), 1301-1310, 2002-08-23

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1363388845291002752
• NII論文ID: 80015480139
• DOI: 10.1126/science.1072104
• ISSN: 10959203
• ISSN: 00368075
• ISSN: http://id.crossref.org/issn/00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.1072104
• データソース種別:
  • Crossref
  • CiNii Articles